Ξ²-trypsin from Bos taurus (bovine)
Enzyme Description
Sequence
IVGGYTCGANTVPYQVSLNSGYHFCGGSLINSQWVVSAAHCYKSGIQVRLGEDNINVVEGNEQFISASKSIVHPSYNSNTLNNDIMLIKLKSAASLNSRVASISLPTSCASAGTQCLISGWGNTKSSGTSYPDVLKCLKAPILSDSSCKSAYPGQITSNMFCAGYLEGGKDSCQGDSGGPVVCSGKLQGIVSWGSGCAQKNKPGVYTKVCNYVSWIKQTIASN
Dayanne Pinho Rosa et al. (2021)
β Evaluation of biological activities, structural and conformational properties of bovine beta- and alpha-trypsin isoforms in aqueous-organic media
International Journal of Biological Macromolecules
Β· doi:10.1016/j.ijbiomac.2021.02.079 β
Β· Activity - Classical Stability - Tm
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Database ID
UniProt: P00760 β
Sequence Annotation
Inferred - from protein name (23 first residues from UniProt sequence absent from the mature protein)
Protein Source
Commercially purchased
Experimental Data (33 measurements)
33 measurements β page 2 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Extraction method | Data type | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Methanol | 70 % (v/v) | 46.0 | 0.0 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Methanol | 80 % (v/v) | 46.0 | 0.0 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Methanol | 90 % (v/v) | 46.0 | 0.0 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Ethanol | 10 % (v/v) | 46.0 | 51.6 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Ethanol | 20 % (v/v) | 46.0 | 56.5 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Ethanol | 30 % (v/v) | 46.0 | 55.5 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Ethanol | 40 % (v/v) | 46.0 | 54.9 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Ethanol | 60 % (v/v) | 46.0 | 3.0 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Stability - Tm | Tm measured by UV-absorption spectroscopy in the presence of organic solvent | Ethanol | 20 % (v/v) | 56.5 | 43.2 | Β°C | Direct | Continuous | 50 mM glycine buffer, 20 mM CaCl2 | 3 | β | β | β | β | β | Classical aqueous control (in Β°C) |
| Stability - Tm | Tm measured by UV-absorption spectroscopy in the presence of organic solvent | Ethanol | 40 % (v/v) | 56.5 | 48.8 | Β°C | Direct | Continuous | 50 mM glycine buffer, 20 mM CaCl2 | 3 | β | β | β | β | β | Classical aqueous control (in Β°C) |
| Stability - Tm | Tm measured by UV-absorption spectroscopy in the presence of organic solvent | Ethanol | 50 % (v/v) | 56.5 | 48.2 | Β°C | Direct | Continuous | 50 mM glycine buffer, 20 mM CaCl2 | 3 | β | β | β | β | β | Classical aqueous control (in Β°C) |
| Stability - Tm | Tm measured by UV-absorption spectroscopy in the presence of organic solvent | Ethanol | 60 % (v/v) | 56.5 | 53.4 | Β°C | Direct | Continuous | 50 mM glycine buffer, 20 mM CaCl2 | 3 | β | β | β | β | β | Classical aqueous control (in Β°C) |
| Stability - Tm | Tm measured by UV-absorption spectroscopy in the presence of organic solvent | Ethanol | 80 % (v/v) | 56.5 | 68.6 | Β°C | Direct | Continuous | 50 mM glycine buffer, 20 mM CaCl2 | 3 | β | β | β | β | β | Classical aqueous control (in Β°C) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume. β β β Reference value. Hover for details.
Visualization : Stability β Tm
One bar per measurement. Colour = solvent, shade = solvent volume. β β β Reference value. Hover for details.