Ξ²-trypsin from Bos taurus (bovine)
Enzyme Description
Sequence
IVGGYTCGANTVPYQVSLNSGYHFCGGSLINSQWVVSAAHCYKSGIQVRLGEDNINVVEGNEQFISASKSIVHPSYNSNTLNNDIMLIKLKSAASLNSRVASISLPTSCASAGTQCLISGWGNTKSSGTSYPDVLKCLKAPILSDSSCKSAYPGQITSNMFCAGYLEGGKDSCQGDSGGPVVCSGKLQGIVSWGSGCAQKNKPGVYTKVCNYVSWIKQTIASN
Dayanne Pinho Rosa et al. (2021)
β Evaluation of biological activities, structural and conformational properties of bovine beta- and alpha-trypsin isoforms in aqueous-organic media
International Journal of Biological Macromolecules
Β· doi:10.1016/j.ijbiomac.2021.02.079 β
Β· Activity - Classical Stability - Tm
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Database ID
UniProt: P00760 β
Sequence Annotation
Inferred - from protein name (23 first residues from UniProt sequence absent from the mature protein)
Protein Source
Commercially purchased
Experimental Data (33 measurements)
33 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Extraction method | Data type | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Methanol | 20 % v/v | 46.0 | 37.4 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | 1-Propanol | 90 % v/v | 46.0 | 0.0 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | 1-Propanol | 80 % v/v | 46.0 | 0.0 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | 1-Propanol | 70 % v/v | 46.0 | 0.0 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | 1-Propanol | 60 % v/v | 46.0 | 0.0 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | 1-Propanol | 50 % v/v | 46.0 | 1.0 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | 1-Propanol | 40 % v/v | 46.0 | 3.9 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | 1-Propanol | 32 % v/v | 46.0 | 25.3 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | 1-Propanol | 30 % v/v | 46.0 | 33.2 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | 1-Propanol | 20 % v/v | 46.0 | 47.7 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | 1-Propanol | 10 % v/v | 46.0 | 50.2 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Ethanol | 90 % v/v | 46.0 | 0.0 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Ethanol | 80 % v/v | 46.0 | 0.0 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Ethanol | 70 % v/v | 46.0 | 0.0 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Ethanol | 50 % v/v | 46.0 | 28.8 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Methanol | 10 % v/v | 46.0 | 38.6 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Methanol | 30 % v/v | 46.0 | 37.7 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Methanol | 40 % v/v | 46.0 | 41.1 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Methanol | 50 % v/v | 46.0 | 21.2 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent | Methanol | 60 % v/v | 46.0 | 4.7 | Β΅M/L | Digital | Continuous | 100 mM Tris-HCl buffer, 20 mM CaCl2 | 8 | 37Β°C | 0.9 mM Benzoyl-DL-arginine p-nitroanilide | N-Benzoyl-L-arginine , p-Nitroaniline | β | β | Classical aqueous control (in microM/L) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Tm
One bar per measurement. Colour = solvent, shade = solvent volume.