Imine reductase from Streptosporangium roseum DSM43021

Enzyme Description

Species
Streptosporangium roseum DSM43021 β†—
Extremophile
No
EC Number
1.5.1.20 β†—

Sequence

Length: 291 amino acids
MRDTDVTVLGLGLMGQALAGAFLKDGHATTVWNRSEGKAGQLAEQGAVLASSARDAAEASPLVVVCVSDHAAVRAVLDPLGDVLAGRVLVNLTSGTSEQARATAEWAAERGITYLDGAIMAIPQVVGTADAFLLYSGPEAAYEAHEPTLRSLGAGTTYLGADHGLSSLYDVALLGIMWGTLNSFLHGAALLGTAKVEATTFAPFANRWIEAVTGFVSAYAGQVDQGAYPALDATIDTHVATVDHLIHESEAAGVNTELPRLVRTLADRALAGGQGGLGYAAMIEQFRSPSA
Philipp N. Scheller et al. (2016) β€” The biochemical characterization of three imine-reducing enzymes from Streptosporangium roseum DSM43021, Streptomyces turgidiscabies and Paenibacillus elgii
Applied Microbiology and Biotechnology  Β· doi:10.1007/s00253-016-7740-0 β†—  Β· Activity - Classical
40 measurements ▼
Database ID
NCBI: WP_012887675.1 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli JW5510

Experimental Data (40 measurements)

40 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 5 % v/v 100 48 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetonitrile 10 % v/v 100 6 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetonitrile 15 % v/v 100 0 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetonitrile 20 % v/v 100 0 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetonitrile 25 % v/v 100 0 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5 % v/v 100 43 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 32 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 15 % v/v 100 18 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 100 11 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 25 % v/v 100 7 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetonitrile 5 % v/v 100 25 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 10 % v/v 100 33 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 15 % v/v 100 17 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 20 % v/v 100 8 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 25 % v/v 100 2 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Glycerol 5 % v/v 100 61 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Glycerol 10 % v/v 100 53 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Glycerol 15 % v/v 100 36 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Glycerol 20 % v/v 100 35 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Glycerol 25 % v/v 100 16 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

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Structure

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