Imine reductase from Paenibacillus elgii

Enzyme Description

Species
Paenibacillus elgii β†—
Extremophile
No
EC Number
1.5.1.20 β†—

Sequence

Length: 309 amino acids
MNSSNPKDNISVGSASTATNRKSVTVMGLGPMGQAMAGVFLESGYEVTVWNRTASKADELVAKGAIRASTVDEALAANELVILSLTDYDAMYAILEPSSANLSGKVLVNLSSDTPEKVREAAKWLADRGARHVTGGVQVPPPSGIGKPESYTYSGPREVFEAHRESLEILTGTDYRGEDPGLAMLYYQIQMDIFWTSMLSYLHALAVAKANGITAKQFLPYASATLSSLPQFVEFYTPRLDEGKHPGDVDRLAMGLASVEHIVHTTEDAGIDTTLPAAVLEIFKRGMENGHAGDSFTSLIEIFKNPVRS
Philipp N. Scheller et al. (2016) β€” The biochemical characterization of three imine-reducing enzymes from Streptosporangium roseum DSM43021, Streptomyces turgidiscabies and Paenibacillus elgii
Applied Microbiology and Biotechnology  Β· doi:10.1007/s00253-016-7740-0 β†—  Β· Activity - Classical
40 measurements ▼
Database ID
NCBI: WP_010497949.1 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli JW5510

Experimental Data (40 measurements)

40 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Glycerol 5 % v/v 100 82 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 10 % v/v 100 31 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 15 % v/v 100 22 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 20 % v/v 100 25 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 25 % v/v 100 11 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5 % v/v 100 50 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 29 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 15 % v/v 100 14 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 100 9 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 25 % v/v 100 4 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 5 % v/v 100 45 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Glycerol 10 % v/v 100 77 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Glycerol 15 % v/v 100 62 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Glycerol 20 % v/v 100 59 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Glycerol 25 % v/v 100 52 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 5 % v/v 100 92 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 10 % v/v 100 91 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 15 % v/v 100 77 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 20 % v/v 100 80 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 25 % v/v 100 52 % Digital Continuous 50 mM sodium phosphate buffer 7 30Β°C 5 mM 2-Methylpyrroline 2-Methylpyrrolidine 0.3 Β΅M NADPH β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

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Structure

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