Cyclohexanone monooxygenase from Thermocrispum municipale

Enzyme Description

Species
Thermocrispum municipale β†—
Extremophile
Yes thermophilic organism
EC Number
1.14.13.22 β†—

Sequence

Length: 541 amino acids
MSTTQTPDLDAIVIGAGFGGIYMLHKLRNDLGLSVRVFEKGGGVGGTWYWNKYPGAKSDTEGFVYRYSFDKELLREYDWTTRYLDQPDVLAYLEHVVERYDLARDIQLNTEVTDAIFDEETELWRVTTAGGETLTARFLVTALGLLSRSNIPDIPGRDSFAGRLVHTNAWPEDLDITGKRVGVIGTGSTGTQFIVAAAKMAEQLTVFQRTPQYCVPSGNGPMDPDEVARIKQNFDSIWDQVRSSTVAFGFEESTVEAMSVSESERQRVFQQAWDKGNGFRFMFGTFCDIATNPEANAAAAAFIRSKIAEIVKDPETARKLTPTDLYAKRPLCNEGYYETYNRDNVSLVSLKETPIEEIVPQGVRTSDGVVHELDVLVFATGFDAVDGNYRAMNLRGRDGRHINEHWTEGPTSYLGVTKAGFPNMFMILGPNGPFTNLPPSIEAQVEWISDLIDKATREGLTTVEPTADAEREWTETCAEIANMTLFPKADSWIFGANIPGKRHAVMFYLGGLGNYRRQLADVADGGYRGFQLRGERAQAVA
Elvira Romero et al. (2016) β€” Characterization and Crystal Structure of a Robust Cyclohexanone Monooxygenase
Angewandte Chemie International Edition  Β· doi:10.1002/anie.201608951 β†—  Β· Activity + Stability - Conversion
5 measurements ▼
Database ID
UniProt: A0A1L1QK40 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli NEB 10-Ξ²

Experimental Data (5 measurements)

5 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity + Stability - Conversion Conversion after incubation (48 hours at 17Β°C) in the presence of organic solvent, measured by gas chromatography Methanol 30 % v/v 100 96 % Direct Continuous 50 mM Tris-HCl buffer, 20 mM phosphite 7 17Β°C 11 mM 2-Butanone Ethyl Acetate 150 Β΅M NADPH 200 rpm Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (48 hours at 17Β°C) in the presence of organic solvent, measured by gas chromatography Ethanol 30 % v/v 100 56 % Direct Continuous 50 mM Tris-HCl buffer, 20 mM phosphite 7 17Β°C 11 mM 2-Butanone Ethyl Acetate 150 Β΅M NADPH 200 rpm Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (48 hours at 17Β°C) in the presence of organic solvent, measured by gas chromatography 1,4-Dioxane 15 % v/v 100 56 % Direct Continuous 50 mM Tris-HCl buffer, 20 mM phosphite 7 17Β°C 11 mM 2-Butanone Ethyl Acetate 150 Β΅M NADPH 200 rpm Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (48 hours at 17Β°C) in the presence of organic solvent, measured by gas chromatography 1,3-Dioxane 15 % v/v 100 51 % Direct Continuous 50 mM Tris-HCl buffer, 20 mM phosphite 7 17Β°C 11 mM 2-Butanone Ethyl Acetate 150 Β΅M NADPH 200 rpm Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (48 hours at 17Β°C) in the presence of organic solvent, measured by gas chromatography 2-Methyl-1,3-Dioxolane 15 % v/v 100 25 % Direct Continuous 50 mM Tris-HCl buffer, 20 mM phosphite 7 17Β°C 11 mM 2-Butanone Ethyl Acetate 150 Β΅M NADPH 200 rpm Classical aqueous control (in %)

Visualization : Activity + Stability β€” Conversion

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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