5'-nucleotidase from Shewanella violacea

Enzyme Description

Species

Shewanella violacea ↗

Extremophile

Yes "psychrophilic" and "piezophilic" organism

EC Number

3.1.3.5 ↗

Sequence

Length: 569 amino acids

MKNKLIKGLVATAVLAALAGCGSDNDDKDVVLPATCAEAGEACTIFTVLHTNDNHGRFFENKYGEYGMAARKTLIDQIRAEVEANGGETILLSGGDINTGVPESDLQDAVPDFIGMNLIGYDAMAVGNHEFDNPLDVVDMQRKLANFPMLAANIYDKVSGTRYFDPYKVFDVNGLRIAVVGLTTEDTAKIANPEFIGGLEFTDPQEELKKVIQDIKDADAADLIFATTHMGHYADAASGSNAPGDVALARAMTAGDLAAVIGGHSQNPVCMEGDAYDAEFTPGGECKPDVQNGTFIMQAHEWGKYVGQANFEYFNDELHLASYKLIPVNLKAKDADGVRQFIGEEITPDATVLEIMQQYQNAGQEELLEVIGTTDGKLEGERAVVRTQQTNLGRLIAEAQRTKVGADFAVMNSGGVRASIEAGEITYKDVLTVQPFGNSITKSTMTGAEITEYLNAVATVDIGETGSGAYPQLVGIKMDVDCEAKTVAINDINGKGYAVDASYSFTVPSFNAAGGDGYPVLTPVMTGYVDAEVMYTFIKDIGDILLADFAPVASDIQFLNSNSTLACQR

Kiko Fujimori et al. (2019) — Differences in biochemical properties of two 5'-nucleotidases from deep- and shallow-sea Shewanella species under various harsh conditions

Bioscience, Biotechnology, and Biochemistry · doi:10.1080/09168451.2019.1578641 ↗ · Activity - Classical

24 measurements ▼

Database ID

UniParc: UPI000311FC99 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Purified, bacterium Shewanella violacea

Experimental Data (24 measurements)

24 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20 % v/v	100.0	28.2	%	Digital	Continuous	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate , Inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10 % v/v	100.0	60.2	%	Digital	Continuous	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate , Inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Glycerol	30 % v/v	100.0	9.5	%	Digital	Continuous	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate , Inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Glycerol	20 % v/v	100.0	45.9	%	Digital	Continuous	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate , Inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)

Activity - Classical

Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent

Ethanol

10 % v/v

100.0

22.4

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Acetone

30 % v/v

100.0

18.7

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Acetone

20 % v/v

100.0

19.4

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Acetone

10 % v/v

100.0

35.0

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Acetonitrile

30 % v/v

100.0

3.1

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Acetonitrile

20 % v/v

100.0

3.1

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Acetonitrile

10 % v/v

100.0

10.2

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Isopropanol

30 % v/v

100.0

4.4

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Isopropanol

20 % v/v

100.0

4.4

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Isopropanol

10 % v/v

100.0

30.9

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Ethanol

30 % v/v

100.0

2.7

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Ethanol

20 % v/v

100.0

3.7

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Glycerol

10 % v/v

100.0

84.4

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Methanol

30 % v/v

100.0

5.1

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Methanol

20 % v/v

100.0

7.8

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Methanol

10 % v/v

100.0

37.4

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Dimethylformamide (DMF)

30 % v/v

100.0

11.6

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Dimethylformamide (DMF)

20 % v/v

100.0

15.3

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Dimethylformamide (DMF)

10 % v/v

100.0

40.8

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Dimethyl Sulfoxide (DMSO)

30 % v/v

100.0

13.9

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Dimethyl Sulfoxide (DMSO)

20 % v/v

100.0

28.2

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Dimethyl Sulfoxide (DMSO)

10 % v/v

100.0

60.2

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Glycerol

30 % v/v

100.0

9.5

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

Activity - Classical

Glycerol

20 % v/v

100.0

45.9

Digital

Continuous

20 mM Tris-HCl buffer

30°C

4 mM ATP (Adenosine triphosphate)

Adenosine 5′-diphosphate, Inorganic phosphate

10 mM MgCl₂

—

Classical aqueous control (in %)

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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5'-nucleotidase from Shewanella violacea

Enzyme Description

Sequence

Experimental Data (24 measurements)

Visualization : Activity — Classical

Structure

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