5'-nucleotidase from Shewanella amazonensis
Enzyme Description
Sequence
MTNKLIKGLIATAVLAALAGCNSDDDKVPTTCAEAGAACKTFTVLHTNDNHGRFWENKDGEYGLAAQKTLVDQIRAEVSKKGGQTLLLSGGDINTGVPESDLQDAIPDFTGMNKIGYDAMAVGNHEFDNPLSIVDMQRSLAEFPMLAANIYRKNAKGEPGERYFDAYKIFDVNGIKVAVIGLTTEDTKKIGNPEYISELFFTDPKTEVAKVIKEIKDAKSADIIFAVTHMGHYADGQNGSNAPGDVALARSLKEGELQVVIGGHSQNPVCMEPGNKAYAAFQPGDACAPDQQNGTWIMQAHEWGKYVGRADFEYFNGELHLASYKLVVPNLVKEVTDEAGNKKKLVVGEKIEPDTELKELLSYYQARGQGQLDEEIADTDGVLEKSRLKQTNLGRLIADAQRTKVGADIGIMNSGGVRASIETGKITYRDVLTVQPFGNMVTLSTKTGAELTEYLGAVASLQIGAGAYAQITGVKMNVDCIAKTVNISEVNGKPFSAINNYTFTVPSFNAAGGDGYPKLNPVQTGYVDAEVLYTFFKNKNQIVAADYNPVGDIVYENSESVEGCKITAK
Kiko Fujimori et al. (2019)
β Differences in biochemical properties of two 5'-nucleotidases from deep- and shallow-sea Shewanella species under various harsh conditions
Bioscience, Biotechnology, and Biochemistry
Β· doi:10.1080/09168451.2019.1578641 β
Β· Activity - Classical
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Database ID
UniParc: UPI0004D6B912 β
Sequence Annotation
Explicit - Provided
Protein Source
Purified, bacterium Shewanella amazonensis
Experimental Data (24 measurements)
24 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Extraction method | Data type | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Ethanol | 10 % v/v | 100.0 | 97.6 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Acetone | 30 % v/v | 100.0 | 30.2 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Acetone | 20 % v/v | 100.0 | 58.8 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Acetone | 10 % v/v | 100.0 | 90.8 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Acetonitrile | 30 % v/v | 100.0 | 16.3 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Acetonitrile | 20 % v/v | 100.0 | 53.4 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Acetonitrile | 10 % v/v | 100.0 | 85.7 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Isopropanol | 30 % v/v | 100.0 | 17.7 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Isopropanol | 20 % v/v | 100.0 | 55.8 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Isopropanol | 10 % v/v | 100.0 | 90.8 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Ethanol | 30 % v/v | 100.0 | 33.7 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Ethanol | 20 % v/v | 100.0 | 69.0 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Glycerol | 10 % v/v | 100.0 | 96.9 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Methanol | 30 % v/v | 100.0 | 43.9 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Methanol | 20 % v/v | 100.0 | 60.9 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Methanol | 10 % v/v | 100.0 | 83.3 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 30 % v/v | 100.0 | 52.0 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 20 % v/v | 100.0 | 77.2 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 10 % v/v | 100.0 | 105.4 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 30 % v/v | 100.0 | 74.1 | % | Digital | Continuous | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , Inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.