Acetylcholinesterase (gene Ache) from Mus musculus (mouse)
Enzyme Description
Sequence
EDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGVLDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPASPTPVLIWIYGGGFYSGAASLDVYDGRFLAQVEGAVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLALQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRATLLARLVGCPPGGAGGNDTELIACLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPEALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPEDPTHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPLDPSLNYTTEERIFAQRLMKYWTNFARTGDPNDPRDSKSPQWPPYTTAAQQYVSLNLKPLEVRRGLRAQTCAFWNRFLPKLLSAT
Noelle A. Novales et al. (2022)
β Comparing the effects of organic cosolvents on acetylcholinesterase and butyrylcholinesterase activity
Analytical Biochemistry
Β· doi:10.1016/j.ab.2022.114796 β
Β· Activity - Classical Activity - Michaelis-Menten
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Database ID
UniProt: P21836 β
Sequence Annotation
Explicit - Provided PDB Accession Number (34 first residues from UniProt sequence absent from the mature protein)
Protein Source
Commercially purchased
Experimental Data (28 measurements)
28 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Extraction method | Data type | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) | Methanol | 2 % v/v | 100.0 | 92.0 | % | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) | Methanol | 1 % v/v | 100.0 | 95.0 | % | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) | Dimethyl Sulfoxide (DMSO) | 5 % v/v | 100.0 | 5.0 | % | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) | Dimethyl Sulfoxide (DMSO) | 2 % v/v | 100.0 | 14.0 | % | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) | Dimethyl Sulfoxide (DMSO) | 1 % v/v | 100.0 | 25.0 | % | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) | Acetone | 5 % v/v | 100.0 | 12.0 | % | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) | Acetone | 2 % v/v | 100.0 | 23.0 | % | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) | Acetone | 1 % v/v | 100.0 | 41.0 | % | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) | Acetonitrile | 5 % v/v | 100.0 | 21.0 | % | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) | Acetonitrile | 2 % v/v | 100.0 | 50.0 | % | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) | Acetonitrile | 1 % v/v | 100.0 | 67.0 | % | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) | Methanol | 5 % v/v | 100.0 | 81.0 | % | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in %) |
| Activity - Michaelis-Menten | Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 5 % v/v | 0.076 | 1.65 | mM | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in mM) |
| Activity - Michaelis-Menten | Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent | Acetone | 5 % v/v | 0.076 | 1.35 | mM | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in mM) |
| Activity - Michaelis-Menten | Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent | Acetonitrile | 5 % v/v | 0.076 | 0.497 | mM | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in mM) |
| Activity - Michaelis-Menten | Km (mM), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent | Methanol | 5 % v/v | 0.076 | 0.155 | mM | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in mM) |
| Activity - Michaelis-Menten | Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 5 % v/v | 133.0 | 58.0 | 10^-8 * mol/s | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in 10^-8 * mol/s) |
| Activity - Michaelis-Menten | Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent | Acetone | 5 % v/v | 133.0 | 78.0 | 10^-8 * mol/s | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in 10^-8 * mol/s) |
| Activity - Michaelis-Menten | Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent | Acetonitrile | 5 % v/v | 133.0 | 91.0 | 10^-8 * mol/s | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in 10^-8 * mol/s) |
| Activity - Michaelis-Menten | Vmax (*10^-8 mol/s), measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of Ellman's reagent reaction product with free thiols 2-nitro-5-thiobenzoate, 412 nm) in the presence of organic solvent | Methanol | 5 % v/v | 133.0 | 135.0 | 10^-8 * mol/s | Direct | Continuous | 100 mM sodium phosphate buffer, 1 mM MgClβ, 0.2 MM Ellman's reagent | 7.5 | 25Β°C | 100 Β΅M Acetylthiocholine | Acetate , Thiocholine | β | β | Classical aqueous control (in 10^-8 * mol/s) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Activity β Michaelis-Menten
One bar per measurement. Colour = solvent, shade = solvent volume.