Transketolase (gene tktA) from Escherichia coli

Enzyme Description

Species
Escherichia coli β†—
Extremophile
No
EC Number
2.2.1.1 β†—

Sequence

Length: 663 amino acids
MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDFLKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPEVGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMMEGISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDGHDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQLGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMPSDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSGSKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAALMKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVERQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVAAYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVGLNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL
Phattaraporn Morris et al. (2021) β€” Biophysical characterization of the inactivation of E. coli transketolase by aqueous co-solvents
Scientific Reports  Β· doi:10.1038/s41598-021-03001-8 β†—  Β· Stability - Incubation
71 measurements ▼
Database ID
UniProt: P27302 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli XL10-Gold

Experimental Data (71 measurements)

71 measurements β€” page 1 of 4
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Isopropanol 10 % v/v 19.3 12.6 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, without TPP or MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Isopropanol 8 % v/v 19.3 11.9 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Isopropanol 6 % v/v 19.3 12.3 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Isopropanol 4 % v/v 19.3 13.2 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Isopropanol 2 % v/v 19.3 14.2 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Isopropanol 30 % v/v 19.3 0.0 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, without TPP or MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Isopropanol 25 % v/v 19.3 0.0 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, without TPP or MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Isopropanol 20 % v/v 19.3 6.7 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, without TPP or MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Isopropanol 15 % v/v 19.3 11.1 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, without TPP or MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Isopropanol 10 % v/v 19.3 10.8 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Isopropanol 8 % v/v 19.3 13.1 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, without TPP or MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Isopropanol 6 % v/v 19.3 13.6 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, without TPP or MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Isopropanol 4 % v/v 19.3 13.8 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, without TPP or MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Isopropanol 2 % v/v 19.3 15.1 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, without TPP or MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Ethyl Acetate 30 % v/v 19.3 0.5 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Ethyl Acetate 25 % v/v 19.3 0.6 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Ethyl Acetate 20 % v/v 19.3 0.8 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Ethyl Acetate 15 % v/v 19.3 1.2 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Tetrahydrofuran (THF) 8 % v/v 19.3 0.0 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, without TPP or MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
Stability - Incubation Activity after incubation (3 hours at 25Β°C), measured by HPLC in aquous conditions Tetrahydrofuran (THF) 10 % v/v 19.3 0.0 Β΅M/mg/mL Digital Continuous Incubation: 50 mM Tris-HCl buffer, with 4.8 mM TPP and 18 mM MgClβ‚‚, Assay: 50 mM Tris-HCl buffer, 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 50 mM Glycoaldehyde, 50 mM Hydroxypyruvic acid Carbon Dioxide (CO2) , Erythrulose Assay: 2.4 mM TPP, 9 mM MgClβ‚‚ Incubation: 1000 rpm Non-incubated control (in microM/mg/mL), assay in aqueous phase | Clear about assay in diluted aqueous phase/Goal of article : comparison residual activity when incubated with and without cofactors
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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