Formate oxidase (gene AnFOX) from Aspergillus nomiae

Enzyme Description

Species
Aspergillus nomiae β†—
Extremophile
No
EC Number
1.17.1.9 β†—

Sequence

Length: 593 amino acids
MSAQVNNNQYDFIVVGGGTAGNAIAGRLSENPSVRVLVVEAGIANSGQIEDITTPSKAFGLRGSQYDWAYKTTMIKRDDYERVEKPNTRGKALGGSSCANYFTWIPGSKATFDDWYEFGGSDWTWDNCVDYLRKCATYHDDEGLYPTDLNKIGTGGPVQISHAELVPELETFRNALTTAWTSKGEPLSEDIYSGEMHGLTHCVDTIYKGQRQGSWLYLENKPNVTILSQVHSKRLIIDRTTQTCTGVTVVDPTNGAELNLYATREVIVSQGVFETPKLLLLSGIGPAAELNKHGIDVVVDSPHVGKNLLDHPIVPFVLRLKDGVALDDHIHRAGPANEAAVAAYRRDKTGPAASGFLELVGFPRIDERLNRYPAYREAKAANGGLDPFGPAGQPHFELDFVGLFSSAFQWHYPVPPSGSYMTVIVDLLRPVSEGGEVTLNSTDALQQPNINLNFFASDLDVLAMREGVRWTYDVLTKGEGFKDLVLEEYPWNMPLDSDEAMNKAVLDRSQTGFHPCGTARLGKNVQQGVVDSKLRVHGIKNLRVADASIMPVIPDCRIQNSVYMIGEKGADIIKAQYRELYEQKNIPYFSSKL
Zhenyu Zhai et al. (2025) β€” Discovery and characterization of high-activity formate oxidase with low Km value under nonacidic conditions
Bioscience, Biotechnology, and Biochemistry  Β· doi:10.1093/bbb/zbaf092 β†—  Β· Activity - Classical
36 measurements ▼
Database ID
UniProt: A0A0L1IR91 β†—
Sequence Annotation
Explicit - Provided UniProt Accesion Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (36 measurements)

36 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Glycerol 20 % v/v 100.0 223.5 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Dichloromethane 10 % v/v 100.0 87.2 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Methanol 10 % v/v 100.0 86.2 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 10 % v/v 100.0 95.9 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Isobutanol 10 % v/v 100.0 92.8 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent 1-Propanol 10 % v/v 100.0 28.1 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 10 % v/v 100.0 33.7 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100.0 174.5 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Dimethylformamide (DMF) 10 % v/v 100.0 127.0 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Glycerol 10 % v/v 100.0 135.7 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Dichloromethane 20 % v/v 100.0 67.3 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Methanol 20 % v/v 100.0 53.1 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 20 % v/v 100.0 49.5 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Isobutanol 20 % v/v 100.0 99.5 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent 1-Propanol 20 % v/v 100.0 0.0 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 20 % v/v 100.0 0.0 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 100.0 142.3 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Dimethylformamide (DMF) 20 % v/v 100.0 44.4 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Glycerol 5 % v/v 100.0 102.5 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Dichloromethane 2 % v/v 100.0 81.1 % Digital Continuous 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

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Structure

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