Levansucrase from Bacillus subtilis

Enzyme Description

Species
Bacillus subtilis β†—
Extremophile
No
EC Number
2.4.1.10 β†—

Sequence

Length: 473 amino acids
MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK
E. Castillo et al. (2004) β€” Synthesis of levan in water-miscible organic solvents
Journal of Biotechnology  Β· doi:10.1016/j.jbiotec.2004.06.003 β†—  Β· Activity - Classical Stability - Incubation Selectivity - Chemoselectivity
38 measurements ▼
Database ID
UniProt: P05655 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, bacterium Bacillus subtilis

Experimental Data (38 measurements)

38 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent Acetonitrile 5 % v/v 100.0 97.0 % Digital Continuous 50 mM phosphate buffer 6 30Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent tert-Butanol 95 % v/v 100.0 0.0 % Digital Continuous 50 mM phosphate buffer 6 37Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent tert-Butanol 90 % v/v 100.0 26.0 % Digital Continuous 50 mM phosphate buffer 6 37Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent tert-Butanol 82.5 % v/v 100.0 90.0 % Digital Continuous 50 mM phosphate buffer 6 37Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent tert-Butanol 75 % v/v 100.0 92.0 % Digital Continuous 50 mM phosphate buffer 6 37Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent tert-Butanol 62.5 % v/v 100.0 94.0 % Digital Continuous 50 mM phosphate buffer 6 37Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent tert-Butanol 50 % v/v 100.0 141.0 % Digital Continuous 50 mM phosphate buffer 6 37Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent tert-Butanol 30 % v/v 100.0 118.0 % Digital Continuous 50 mM phosphate buffer 6 37Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent tert-Butanol 15 % v/v 100.0 87.0 % Digital Continuous 50 mM phosphate buffer 6 37Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent Acetone 50 % v/v 100.0 76.0 % Digital Continuous 50 mM phosphate buffer 6 30Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent Acetonitrile 50 % v/v 100.0 79.0 % Digital Continuous 50 mM phosphate buffer 6 30Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent tert-Butanol 50 % v/v 100.0 137.0 % Digital Continuous 50 mM phosphate buffer 6 30Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent Acetone 40 % v/v 100.0 79.0 % Digital Continuous 50 mM phosphate buffer 6 30Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent tert-Butanol 40 % v/v 100.0 136.0 % Digital Continuous 50 mM phosphate buffer 6 30Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent tert-Butanol 5 % v/v 100.0 97.0 % Digital Continuous 50 mM phosphate buffer 6 30Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent Acetone 5 % v/v 100.0 99.0 % Digital Continuous 50 mM phosphate buffer 6 30Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent tert-Butanol 10 % v/v 100.0 95.0 % Digital Continuous 50 mM phosphate buffer 6 30Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent Acetonitrile 10 % v/v 100.0 98.0 % Digital Continuous 50 mM phosphate buffer 6 30Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent Acetone 10 % v/v 100.0 91.0 % Digital Continuous 50 mM phosphate buffer 6 30Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent tert-Butanol 20 % v/v 100.0 121.0 % Digital Continuous 50 mM phosphate buffer 6 30Β°C 100 g/L Sucrose Reducing sugars β€” β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Selectivity - Chemoselectivity

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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