Inorganic pyrophosphatase from Saccharomyces cerevisiae

Enzyme Description

Species
Saccharomyces cerevisiae β†—
Extremophile
No
EC Number
3.6.1.1 β†—

Sequence

Length: 287 amino acids
MTYTTRQIGAKNTLEYKVYIEKDGKPVSAFHDIPLYADKENNIFNMVVEIPRWTNAKLEITKEETLNPIIQDTKKGKLRFVRNCFPHHGYIHNYGAFPQTWEDPNVSHPETKAVGDNDPIDVLEIGETIAYTGQVKQVKALGIMALLDEGETDWKVIAIDINDPLAPKLNDIEDVEKYFPGLLRATNEWFRIYKIPDGKPENQFAFSGEAKNKKYALDIIKETHDSWKQLIAGKSSDSKGIDLTNVTLPDTPTYSKAASDAIPPASPKADAPIDKSIDKWFFISGSV
Rodrigo Grazinoli-Garrido et al. (2004) β€” Inactivation of yeast inorganic pyrophosphatase by organic solvents
Anais da Academia Brasileira de CieΜ‚ncias  Β· doi:10.1590/s0001-37652004000400006 β†—  Β· Activity - Classical
50 measurements ▼
Database ID
UniProt: P00817 β†—
Sequence Annotation
Inferred
Protein Source
Commercially purchased

Experimental Data (50 measurements)

50 measurements β€” page 2 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Acetone 0.25 Molar 1700 1762 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 37Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Acetone 0.7 Molar 1700 1617 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 37Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Acetone 1.4 Molar 1700 1498 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 37Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Acetone 2.1 Molar 1700 1242 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 37Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Acetone 2.8 Molar 1700 970 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 37Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Ethanol 2.5 Molar 172 75 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 5Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Methanol 1.25 Molar 172 159 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 5Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Methanol 2.5 Molar 172 132 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 5Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Methanol 3.75 Molar 172 98 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 5Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Methanol 5 Molar 172 71 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 5Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Methanol 0.5 Molar 1700 1603 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 37Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Methanol 1.25 Molar 1700 1507 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 37Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Methanol 2.5 Molar 1700 1397 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 37Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Methanol 3.75 Molar 1700 1137 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 37Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Methanol 5 Molar 1700 945 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 37Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Ethanol 0.33 Molar 172 164 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 5Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Ethanol 0.85 Molar 172 141 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 5Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Ethanol 1.7 Molar 172 109 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 5Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Methanol 0.5 Molar 172 162 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 5Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Ethanol 3.4 Molar 172 45 pyrophosphate concentration/(mg*min) Digital Continuous 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 5Β°C 2 mM Tetrasodium pyrophosphate Inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
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Visualization : Activity β€” Classical

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Structure

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