Luciferase from Oplophorus gracilirostris (luminous shrimp)

Enzyme Description

Species

Oplophorus gracilirostris (luminous shrimp) ↗

Extremophile

EC Number

1.13.12.13 ↗

Sequence

Length: 169 amino acids

FTLADFVGDWQQTAGYNQDQVLEQGGLSSLFQALGVSVTPIQKVVLSGENGLKADIHVIIPYEGLSGFQMGLIEMIFKVVYPVDDHHFKIILHYGTLVIDGVTPNMIDYFGRPYPGIAVFDGKQITVTGTLWNGNKIYDERLINPDGSLLFRVTINGVTGWRLCENILA

Satoshi Inouye et al. (2007) — Overexpression, purification and characterization of the catalytic component of Oplophorus luciferase in the deep-sea shrimp, Oplophorus gracilirostris

Protein Expression and Purification · doi:10.1016/j.pep.2007.08.002 ↗ · Activity - Classical

63 measurements ▼

Database ID

UniProt: Q9GV45 ↗

Sequence Annotation

Inferred - from protein name (27 first residues from UniProt sequence absent from the mature protein)

Protein Source

Recombinant, host bacterium Escherichia coli BL21

Experimental Data (63 measurements)

63 measurements — page 2 of 4

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	50 % v/v	100	0	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide , Carbon Dioxide (CO2) , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	100 % v/v	100	0	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide , Carbon Dioxide (CO2) , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	2-Mercaptoethanol	1 % v/v	100	322	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide , Carbon Dioxide (CO2) , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	2-Mercaptoethanol	3 % v/v	100	491	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide , Carbon Dioxide (CO2) , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	2-Mercaptoethanol	5 % v/v	100	282	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide , Carbon Dioxide (CO2) , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Acetone	5 % v/v	100	256	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide , Carbon Dioxide (CO2) , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Acetone	10 % v/v	100	173	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide , Carbon Dioxide (CO2) , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Acetone	15 % v/v	100	16	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide , Carbon Dioxide (CO2) , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Dimethyl Sulfoxide (DMSO)	5 % v/v	100	173	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide , Carbon Dioxide (CO2) , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Dimethyl Sulfoxide (DMSO)	10 % v/v	100	247	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide , Carbon Dioxide (CO2) , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Dimethyl Sulfoxide (DMSO)	15 % v/v	100	300	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide , Carbon Dioxide (CO2) , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Dimethyl Sulfoxide (DMSO)	20 % v/v	100	120	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide , Carbon Dioxide (CO2) , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	10 % v/v	100	0	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2) , coelenteramide , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	2 % v/v	100	84	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2) , coelenteramide , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	3 % v/v	100	84	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2) , coelenteramide , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	5 % v/v	100	106	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2) , coelenteramide , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	10 % v/v	100	90	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2) , coelenteramide , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	15 % v/v	100	31	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2) , coelenteramide , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Ethanol	1 % v/v	100	93	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2) , coelenteramide , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Ethanol	3 % v/v	100	77	%	Digital	Continuous	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2) , coelenteramide , photon	—	—	Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

4 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Methanol

3 % v/v

100

167

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Methanol

5 % v/v

100

293

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Methanol

10 % v/v

100

469

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Methanol

15 % v/v

100

333

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Methanol

20 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Methanol

30 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Ethanol

1 % v/v

100

162

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Ethanol

2 % v/v

100

176

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Ethanol

3 % v/v

100

260

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Ethanol

5 % v/v

100

318

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Ethanol

10 % v/v

100

216

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Ethanol

15 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

0.5 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

1 % v/v

100

209

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

2 % v/v

100

327

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Methanol

2 % v/v

100

160

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

5 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

10 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

30 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

50 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

100 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

2-Mercaptoethanol

1 % v/v

100

322

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

2-Mercaptoethanol

3 % v/v

100

491

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

2-Mercaptoethanol

5 % v/v

100

282

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Acetone

5 % v/v

100

256

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Acetone

10 % v/v

100

173

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Acetone

15 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Dimethyl Sulfoxide (DMSO)

5 % v/v

100

173

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

247

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Dimethyl Sulfoxide (DMSO)

15 % v/v

100

300

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Dimethyl Sulfoxide (DMSO)

20 % v/v

100

120

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

10 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Methanol

2 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Methanol

3 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Methanol

5 % v/v

100

106

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Methanol

10 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Methanol

15 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Ethanol

1 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Ethanol

3 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Ethanol

5 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Ethanol

10 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Ethanol

15 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

1 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

2 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

3 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

4 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

5 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Methanol

1 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

15 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

30 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

1-Butanol

50 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

2-Mercaptoethanol

1 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

2-Mercaptoethanol

3 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

2-Mercaptoethanol

5 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

2-Mercaptoethanol

10 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Acetone

5 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Acetone

10 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Dimethyl Sulfoxide (DMSO)

5 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Dimethyl Sulfoxide (DMSO)

15 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Dimethyl Sulfoxide (DMSO)

20 % v/v

100

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Coelenterazine, ? mM O₂

Carbon Dioxide (CO2), coelenteramide, photon

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)

Methanol

1 % v/v

100

124

Digital

Continuous

30 mM Tris-HCl buffer, 10 mM EDTA

7.6

25°C

1 µg/µL Bis-coelenterazine

Bis-coelenteramide, Carbon Dioxide (CO2), photon

—

Classical aqueous control (in %)

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Luciferase from Oplophorus gracilirostris (luminous shrimp)

Enzyme Description

Sequence

Experimental Data (63 measurements)

Visualization : Activity — Classical

Structure

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