Carboxylesterase (gene BioHe) from Escherichia coli

Enzyme Description

Species
Escherichia coli K12 β†—
Extremophile
No
EC Number
3.1.1.85 β†—

Sequence

Length: 256 amino acids
MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHLVDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQALVTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARALKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPMLDKLWPHSESYIFAKAAHAPFISHPAEFCHLLVALKQRV
Min-A Kwon et al. (2009) β€” Gene Cloning, Expression, and Characterization of a New Carboxylesterase from Serratia sp. SES-01: Comparison with Escherichia coli BioHe Enzyme
Journal of Microbiology and Biotechnology  Β· doi:10.4014/jmb.0804.287 β†—  Β· Activity + Stability - Incubation
21 measurements ▼
Database ID
UniProt: P13001 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 JM109

Experimental Data (21 measurements)

21 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent 1-Butanol 20 % v/v 100 51 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30 % v/v 100 100 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetone 30 % v/v 100 85 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 30 % v/v 100 11 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent 1-Butanol 30 % v/v 100 38 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 30 % v/v 100 30 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 30 % v/v 100 84 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 30 % v/v 100 93 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20 % v/v 100 100 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetone 20 % v/v 100 92 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 20 % v/v 100 37 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 10 % v/v 100 100 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 20 % v/v 100 89 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 20 % v/v 100 91 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 20 % v/v 100 91 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10 % v/v 100 101 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetone 10 % v/v 100 94 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 10 % v/v 100 93 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent 1-Butanol 10 % v/v 100 89 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at 20Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 10 % v/v 100 91 % Direct Continuous Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol Incubation: ?, Assay: 8.5 Incubation: 20Β°C, Assay: 45Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent
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Visualization : Activity + Stability β€” Incubation

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Structure

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