Carboxylesterase (gene BioHe) from Escherichia coli

Enzyme Description

Species

Extremophile

EC Number

3.1.1.85 ↗

Sequence

Length: 256 amino acids

MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHLVDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQALVTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARALKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPMLDKLWPHSESYIFAKAAHAPFISHPAEFCHLLVALKQRV

Min-A Kwon et al. (2009) — Gene Cloning, Expression, and Characterization of a New Carboxylesterase from Serratia sp. SES-01: Comparison with Escherichia coli BioHe Enzyme

Journal of Microbiology and Biotechnology · doi:10.4014/jmb.0804.287 ↗ · Activity + Stability - Incubation

21 measurements ▼

Database ID

UniProt: P13001 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 JM109

Experimental Data (21 measurements)

21 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity + Stability - Incubation	Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	10 % v/v	100	97	%	Direct	Continuous	Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol	Incubation: ?, Assay: 8.5	Incubation: 20°C, Assay: 45°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

1-Butanol

20 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Acetone

30 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

1-Butanol

30 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Isopropanol

30 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Ethanol

30 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Methanol

30 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Acetone

20 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Acetonitrile

20 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Methanol

10 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Isopropanol

20 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Methanol

20 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

101

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Acetone

10 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Acetonitrile

10 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

1-Butanol

10 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Isopropanol

10 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 20°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

Direct

Continuous

Incubation: 50 mM phosphate buffer, Assay: 40 mM Tris-HCl, 12 mM phosphate buffer, 0.8% acetonitrile and 3.2% Ethanol

Incubation: ?, Assay: 8.5

Incubation: 20°C, Assay: 45°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), residual activity, activity measured in the presence of organic solvent

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Visualization : Activity + Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Carboxylesterase (gene BioHe) from Escherichia coli

Enzyme Description

Sequence

Experimental Data (21 measurements)

Visualization : Activity + Stability — Incubation

Structure

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