Polyphenol oxidase from Agaricus bisporus

Enzyme Description

Species

Extremophile

EC Number

1.14.18.1 ↗

Sequence

Length: 568 amino acids

MSHLLVSPLGGGVQPRLEINNFVKNDRQFSLYVQALDRMYATPQNETASYFQVAGVHGYPLIPFDDAVGPTEFSPFDQWTGYCTHGSTLFPTWHRPYVLILEQILSGHAQQIADTYTVNKSEWKKAATEFRHPYWDWASNSVPPPEVISLPKVTITTPNGQKTSVANPLMRYTFNSVNDGGFYGPYNQWDTTLRQPDSTGVNAKDNVNRLKSVLKNAQASLTRATYDMFNRVTTWPHFSSHTPASGGSTSNSIEAIHDNIHVLVGGNGHMSDPSVAPFDPIFFLHHANVDRLIALWSAIRYDVWTSPGDAQFGTYTLRYKQSVDESTDLAPWWKTQNEYWKSNELRSTESLGYTYPEFVGLDMYNKDAVNKTISRKVAQLYGPQRGGQRSLVEDLSNSHARRSQRPAKRSRLGQLLKGLFSDWSAQIKFNRHEVGQSFSVCLFLGNVPEDPREWLVSPNLVGARHAFVRSVKTDHVAEEIGFIPINQWIAEHTGLPSFAVDLVKPLLAQGLQWRVLLADGTPAELDSLEVTILEVPSELTDDEPNPRSRPPRYHKDITHGKRGGCREA

Vered Shuster et al. (2009) — Isolation, Cloning and Characterization of a Tyrosinase with Improved Activity in Organic Solvents from Bacillus megaterium ;

Microbial Physiology · doi:10.1159/000233506 ↗ · Activity - Classical Stability - Incubation

46 measurements ▼

Database ID

UniProt: Q00024 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Commercially purchased

Experimental Data (46 measurements)

46 measurements — page 1 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	20 % v/v	100	35	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50 % v/v	100	6	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40 % v/v	100	21	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30 % v/v	100	66	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20 % v/v	100	84	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10 % v/v	100	85	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	50 % v/v	100	19	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	40 % v/v	100	41	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	30 % v/v	100	48	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	20 % v/v	100	57	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	10 % v/v	100	78	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Isopropanol	50 % v/v	100	29	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Isopropanol	40 % v/v	100	44	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Isopropanol	20 % v/v	100	87	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Isopropanol	30 % v/v	100	52	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	10 % v/v	100	65	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	30 % v/v	100	20	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	40 % v/v	100	12	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	50 % v/v	100	10	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Methanol	10 % v/v	100	48	%	Digital	Continuous	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Acetone

50 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Acetone

40 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Acetone

30 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Acetone

20 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Acetone

10 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Isopropanol

50 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Isopropanol

40 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Isopropanol

20 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Isopropanol

30 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Ethanol

30 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Ethanol

40 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Ethanol

50 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Methanol

10 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Methanol

20 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Methanol

30 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Methanol

40 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Methanol

50 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent

Isopropanol

10 % v/v

100

Digital

Continuous

50 mM potassium phosphate buffer

25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Classical aqueous control (in %)

Stability - Incubation

Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

70 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Water-incubated control (in %) | Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

60 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

50 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

40 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

30 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

20 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

10 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Methanol

70 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Methanol

60 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Methanol

50 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Methanol

40 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Methanol

30 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Methanol

20 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Methanol

10 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Ethanol

70 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Ethanol

60 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Ethanol

50 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Ethanol

40 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Ethanol

30 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Ethanol

20 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

Stability - Incubation

Ethanol

10 % v/v

Digital

Continuous

Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer

7 buffer, 7 assay

Incubation: 30°C, Assay: 25°C

1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)

Dopaquinone

—

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Polyphenol oxidase from Agaricus bisporus

Enzyme Description

Sequence

Experimental Data (46 measurements)

Visualization : Activity — Classical

Visualization : Stability — Incubation

Structure

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