Polyphenol oxidase from Agaricus bisporus
Enzyme Description
Sequence
MSHLLVSPLGGGVQPRLEINNFVKNDRQFSLYVQALDRMYATPQNETASYFQVAGVHGYPLIPFDDAVGPTEFSPFDQWTGYCTHGSTLFPTWHRPYVLILEQILSGHAQQIADTYTVNKSEWKKAATEFRHPYWDWASNSVPPPEVISLPKVTITTPNGQKTSVANPLMRYTFNSVNDGGFYGPYNQWDTTLRQPDSTGVNAKDNVNRLKSVLKNAQASLTRATYDMFNRVTTWPHFSSHTPASGGSTSNSIEAIHDNIHVLVGGNGHMSDPSVAPFDPIFFLHHANVDRLIALWSAIRYDVWTSPGDAQFGTYTLRYKQSVDESTDLAPWWKTQNEYWKSNELRSTESLGYTYPEFVGLDMYNKDAVNKTISRKVAQLYGPQRGGQRSLVEDLSNSHARRSQRPAKRSRLGQLLKGLFSDWSAQIKFNRHEVGQSFSVCLFLGNVPEDPREWLVSPNLVGARHAFVRSVKTDHVAEEIGFIPINQWIAEHTGLPSFAVDLVKPLLAQGLQWRVLLADGTPAELDSLEVTILEVPSELTDDEPNPRSRPPRYHKDITHGKRGGCREA
Vered Shuster et al. (2009)
β Isolation, Cloning and Characterization of a Tyrosinase with Improved Activity in Organic Solvents from Bacillus megaterium ;
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Database ID
UniProt: Q00024 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Commercially purchased
Experimental Data (46 measurements)
46 measurements β page 2 of 3
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Extraction method | Data type | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent | Methanol | 20 % v/v | 100 | 38 | % | Digital | Continuous | 50 mM potassium phosphate buffer | 7 | 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent | Methanol | 30 % v/v | 100 | 35 | % | Digital | Continuous | 50 mM potassium phosphate buffer | 7 | 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent | Methanol | 40 % v/v | 100 | 28 | % | Digital | Continuous | 50 mM potassium phosphate buffer | 7 | 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent | Methanol | 50 % v/v | 100 | 13 | % | Digital | Continuous | 50 mM potassium phosphate buffer | 7 | 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent | Isopropanol | 10 % v/v | 100 | 97 | % | Digital | Continuous | 50 mM potassium phosphate buffer | 7 | 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Classical aqueous control (in %) |
| Stability - Incubation | Activity after incubation (17 hours at 30Β°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 70 % v/v | 61 | 0 | % | Digital | Continuous | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer | 7 buffer, 7 assay | Incubation: 30Β°C, Assay: 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Water-incubated control (in %) | Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided) |
| Stability - Incubation | Activity after incubation (17 hours at 30Β°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 60 % v/v | 61 | 0 | % | Digital | Continuous | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer | 7 buffer, 7 assay | Incubation: 30Β°C, Assay: 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Water-incubated control (in %) | Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided) |
| Stability - Incubation | Activity after incubation (17 hours at 30Β°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 50 % v/v | 61 | 0 | % | Digital | Continuous | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer | 7 buffer, 7 assay | Incubation: 30Β°C, Assay: 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Water-incubated control (in %) | Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided) |
| Stability - Incubation | Activity after incubation (17 hours at 30Β°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 40 % v/v | 61 | 0 | % | Digital | Continuous | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer | 7 buffer, 7 assay | Incubation: 30Β°C, Assay: 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Water-incubated control (in %) | Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided) |
| Stability - Incubation | Activity after incubation (17 hours at 30Β°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 30 % v/v | 61 | 27 | % | Digital | Continuous | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer | 7 buffer, 7 assay | Incubation: 30Β°C, Assay: 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Water-incubated control (in %) | Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided) |
| Stability - Incubation | Activity after incubation (17 hours at 30Β°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20 % v/v | 61 | 57 | % | Digital | Continuous | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer | 7 buffer, 7 assay | Incubation: 30Β°C, Assay: 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Water-incubated control (in %) | Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided) |
| Stability - Incubation | Activity after incubation (17 hours at 30Β°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10 % v/v | 61 | 61 | % | Digital | Continuous | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer | 7 buffer, 7 assay | Incubation: 30Β°C, Assay: 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Water-incubated control (in %) | Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided) |
| Stability - Incubation | Activity after incubation (17 hours at 30Β°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent | Methanol | 70 % v/v | 61 | 0 | % | Digital | Continuous | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer | 7 buffer, 7 assay | Incubation: 30Β°C, Assay: 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Water-incubated control (in %) | Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided) |
| Stability - Incubation | Activity after incubation (17 hours at 30Β°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent | Methanol | 60 % v/v | 61 | 0 | % | Digital | Continuous | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer | 7 buffer, 7 assay | Incubation: 30Β°C, Assay: 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Water-incubated control (in %) | Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided) |
| Stability - Incubation | Activity after incubation (17 hours at 30Β°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent | Methanol | 50 % v/v | 61 | 0 | % | Digital | Continuous | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer | 7 buffer, 7 assay | Incubation: 30Β°C, Assay: 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Water-incubated control (in %) | Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided) |
| Stability - Incubation | Activity after incubation (17 hours at 30Β°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent | Methanol | 40 % v/v | 61 | 0 | % | Digital | Continuous | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer | 7 buffer, 7 assay | Incubation: 30Β°C, Assay: 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Water-incubated control (in %) | Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided) |
| Stability - Incubation | Activity after incubation (17 hours at 30Β°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent | Methanol | 30 % v/v | 61 | 21 | % | Digital | Continuous | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer | 7 buffer, 7 assay | Incubation: 30Β°C, Assay: 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Water-incubated control (in %) | Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided) |
| Stability - Incubation | Activity after incubation (17 hours at 30Β°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent | Methanol | 20 % v/v | 61 | 37 | % | Digital | Continuous | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer | 7 buffer, 7 assay | Incubation: 30Β°C, Assay: 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Water-incubated control (in %) | Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided) |
| Stability - Incubation | Activity after incubation (17 hours at 30Β°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent | Methanol | 10 % v/v | 61 | 48 | % | Digital | Continuous | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer | 7 buffer, 7 assay | Incubation: 30Β°C, Assay: 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Water-incubated control (in %) | Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided) |
| Stability - Incubation | Activity after incubation (17 hours at 30Β°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent | Ethanol | 70 % v/v | 61 | 0 | % | Digital | Continuous | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer | 7 buffer, 7 assay | Incubation: 30Β°C, Assay: 25Β°C | 1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine) | Dopaquinone | β | β | Water-incubated control (in %) | Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.