Feruloyl esterase C (gene faeC) from Talaromyces stipitatus

Enzyme Description

Species

Extremophile

EC Number

3.1.1.73 ↗

Sequence

Length: 530 amino acids

MMLTSAILLLTLGVQLSHADDSSRENFSNRCDQLAKEIHIPNVTVNFVEYVANGTNVTLADNPPSCGQSNQVVLADLCRVAMEVTTSNQSQITLEAWFPENYTGRFLSTGNGGLAGCIQYVDMAYASSMGFATVGANGGHNGTSGESFYHNPDIVEDLSWRSVHTGVVVGKELTKKFYHEGFHKSYYLGCSTGGRQGFKAVQEFVHDFDGVVAGCPAFNFVNLNSWSGHFYPITGNSSADTFLTTAQWTLVQQSVMEQCDSLDGAVDGVIEAIDQCHPVFEQLICRPGQNASECLTGKQVNTAQLVLSPIYGTKGEFLYPRMQPGVENVDMYITYNGDPFAYSTDWYKYVVFSDPNWDPATLNAQDYEIALAQNPSNIQTFEGDLSAFRDAGAKVLTYHGTADPIITGETSKVYYRHVAETMNAAPEELDEFYRYFRIGGMSHCGGGTGATAIGNVLSAQWSNDPDANVLMAMVRWVEEGVAPEYIRGASLGSGPGAKVEYTRRHCKYPTRNVYVGPGNWTDENAWKCIL

Craig B. Faulds et al. (2011) — Influence of organic co-solvents on the activity and substrate specificity of feruloyl esterases

Bioresource Technology · doi:10.1016/j.biortech.2011.01.088 ↗ · Activity - Classical Activity - Michaelis-Menten Stability - C50 Stability - Incubation Activity + Stability - Incubation

133 measurements ▼

Database ID

UniProt: B8LV47 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Recombinant, host yeast Pichia pastoris

Experimental Data (133 measurements)

133 measurements — page 6 of 7

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Michaelis-Menten	Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1 % v/v	12621.0	8301.0	L/(min * mg)	Direct	Continuous	100 mM MOPS	6	37°C	Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in L/(min * mg))
Activity - Michaelis-Menten	Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	3 % v/v	12621.0	7456.0	L/(min * mg)	Direct	Continuous	100 mM MOPS	6	37°C	Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in L/(min * mg))
Activity - Michaelis-Menten	Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5 % v/v	12621.0	9064.0	L/(min * mg)	Direct	Continuous	100 mM MOPS	6	37°C	Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in L/(min * mg))
Activity - Michaelis-Menten	Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10 % v/v	12621.0	6218.0	L/(min * mg)	Direct	Continuous	100 mM MOPS	6	37°C	Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in L/(min * mg))
Activity - Michaelis-Menten	Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15 % v/v	12621.0	5213.0	L/(min * mg)	Direct	Continuous	100 mM MOPS	6	37°C	Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in L/(min * mg))
Activity - Michaelis-Menten	Km (in µM) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1 % v/v	5.61	5.74	µM	Direct	Continuous	100 mM MOPS	6	37°C	Methyl p-coumarate	Methanol , p-Coumaric acid	—	—	Classical aqueous control (in microM)
Activity - Michaelis-Menten	Km (in µM) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	3 % v/v	5.61	6.81	µM	Direct	Continuous	100 mM MOPS	6	37°C	Methyl p-coumarate	Methanol , p-Coumaric acid	—	—	Classical aqueous control (in microM)
Activity - Michaelis-Menten	Km (in µM) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5 % v/v	5.61	15.69	µM	Direct	Continuous	100 mM MOPS	6	37°C	Methyl p-coumarate	Methanol , p-Coumaric acid	—	—	Classical aqueous control (in microM)
Activity - Michaelis-Menten	Km (in µM) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10 % v/v	5.61	33.2	µM	Direct	Continuous	100 mM MOPS	6	37°C	Methyl p-coumarate	Methanol , p-Coumaric acid	—	—	Classical aqueous control (in microM)
Activity - Michaelis-Menten	Km (in µM) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15 % v/v	5.61	43.49	µM	Direct	Continuous	100 mM MOPS	6	37°C	Methyl p-coumarate	Methanol , p-Coumaric acid	—	—	Classical aqueous control (in microM)
Activity - Michaelis-Menten	Vmax (in U/mg) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1 % v/v	19315.0	19513.0	U/mg	Direct	Continuous	100 mM MOPS	6	37°C	Methyl p-coumarate	Methanol , p-Coumaric acid	—	—	Classical aqueous control (in U/mg)
Activity - Michaelis-Menten	Vmax (in U/mg) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	3 % v/v	19315.0	23445.0	U/mg	Direct	Continuous	100 mM MOPS	6	37°C	Methyl p-coumarate	Methanol , p-Coumaric acid	—	—	Classical aqueous control (in U/mg)
Activity - Michaelis-Menten	Vmax (in U/mg) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5 % v/v	19315.0	42835.0	U/mg	Direct	Continuous	100 mM MOPS	6	37°C	Methyl p-coumarate	Methanol , p-Coumaric acid	—	—	Classical aqueous control (in U/mg)
Activity - Michaelis-Menten	Vmax (in U/mg) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10 % v/v	19315.0	55605.0	U/mg	Direct	Continuous	100 mM MOPS	6	37°C	Methyl p-coumarate	Methanol , p-Coumaric acid	—	—	Classical aqueous control (in U/mg)
Activity - Michaelis-Menten	Vmax (in U/mg) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15 % v/v	19315.0	54254.0	U/mg	Direct	Continuous	100 mM MOPS	6	37°C	Methyl p-coumarate	Methanol , p-Coumaric acid	—	—	Classical aqueous control (in U/mg)
Activity - Michaelis-Menten	Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1 % v/v	3443.0	3399.0	L/(min * mg)	Direct	Continuous	100 mM MOPS	6	37°C	Methyl p-coumarate	Methanol , p-Coumaric acid	—	—	Classical aqueous control (in L/(min * mg))
Activity - Michaelis-Menten	Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	3 % v/v	3443.0	3443.0	L/(min * mg)	Direct	Continuous	100 mM MOPS	6	37°C	Methyl p-coumarate	Methanol , p-Coumaric acid	—	—	Classical aqueous control (in L/(min * mg))
Activity - Michaelis-Menten	Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5 % v/v	3443.0	2730.0	L/(min * mg)	Direct	Continuous	100 mM MOPS	6	37°C	Methyl p-coumarate	Methanol , p-Coumaric acid	—	—	Classical aqueous control (in L/(min * mg))
Activity - Michaelis-Menten	Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10 % v/v	3443.0	1675.0	L/(min * mg)	Direct	Continuous	100 mM MOPS	6	37°C	Methyl p-coumarate	Methanol , p-Coumaric acid	—	—	Classical aqueous control (in L/(min * mg))
Activity - Michaelis-Menten	Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15 % v/v	3443.0	1248.0	L/(min * mg)	Direct	Continuous	100 mM MOPS	6	37°C	Methyl p-coumarate	Methanol , p-Coumaric acid	—	—	Classical aqueous control (in L/(min * mg))

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

1 % v/v

100.0

95.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

5 % v/v

100.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100.0

107.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15 % v/v

100.0

105.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25 % v/v

100.0

80.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30 % v/v

100.0

64.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40 % v/v

100.0

19.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

1 % v/v

100.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

5 % v/v

100.0

96.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

10 % v/v

100.0

86.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

15 % v/v

100.0

57.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

20 % v/v

100.0

46.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

25 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1,4-Dioxane

1 % v/v

100.0

97.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1,4-Dioxane

5 % v/v

100.0

84.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1,4-Dioxane

10 % v/v

100.0

63.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1,4-Dioxane

15 % v/v

100.0

34.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1,4-Dioxane

20 % v/v

100.0

14.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1,4-Dioxane

25 % v/v

100.0

6.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1,4-Dioxane

30 % v/v

100.0

4.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Methanol

2 % v/v

100.0

104.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Ethanol

2 % v/v

100.0

110.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1-Propanol

2 % v/v

100.0

101.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Methanol

5 % v/v

100.0

95.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Ethanol

5 % v/v

100.0

102.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1-Propanol

5 % v/v

100.0

86.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Methanol

10 % v/v

100.0

72.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Ethanol

10 % v/v

100.0

81.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1-Propanol

10 % v/v

100.0

63.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

1 % v/v

100.0

89.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

5 % v/v

100.0

86.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100.0

84.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15 % v/v

100.0

71.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100.0

57.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25 % v/v

100.0

37.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30 % v/v

100.0

28.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

35 % v/v

100.0

11.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40 % v/v

100.0

1.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

1 % v/v

100.0

88.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

5 % v/v

100.0

59.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

10 % v/v

100.0

31.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

15 % v/v

100.0

8.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

20 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

25 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

30 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

40 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

1 % v/v

100.0

107.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

5 % v/v

100.0

120.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100.0

101.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15 % v/v

100.0

89.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100.0

42.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25 % v/v

100.0

31.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30 % v/v

100.0

18.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

35 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

1 % v/v

100.0

94.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

5 % v/v

100.0

90.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

10 % v/v

100.0

93.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

15 % v/v

100.0

89.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

20 % v/v

100.0

78.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

25 % v/v

100.0

69.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

30 % v/v

100.0

63.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

35 % v/v

100.0

60.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

40 % v/v

100.0

53.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

50 % v/v

100.0

36.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

75 % v/v

100.0

9.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

98 % v/v

100.0

4.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

5 % v/v

100.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

10 % v/v

100.0

94.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

15 % v/v

100.0

83.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

20 % v/v

100.0

63.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

25 % v/v

100.0

60.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

30 % v/v

100.0

50.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

35 % v/v

100.0

38.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

40 % v/v

100.0

29.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Glycerol

50 % v/v

100.0

13.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

2 % v/v

100.0

90.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

5 % v/v

100.0

87.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100.0

85.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15 % v/v

100.0

72.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100.0

59.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25 % v/v

100.0

38.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30 % v/v

100.0

29.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

35 % v/v

100.0

13.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40 % v/v

100.0

3.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

2 % v/v

100.0

89.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Michaelis-Menten

Km (in µM) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

1 % v/v

2.22

3.4

µM

Direct

Continuous

100 mM MOPS

37°C

Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in µM)

Activity - Michaelis-Menten

Km (in µM) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

3 % v/v

2.22

4.28

µM

Direct

Continuous

100 mM MOPS

37°C

Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in µM)

Activity - Michaelis-Menten

Km (in µM) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

5 % v/v

2.22

4.14

µM

Direct

Continuous

100 mM MOPS

37°C

Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in µM)

Activity - Michaelis-Menten

Km (in µM) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

2.22

7.29

µM

Direct

Continuous

100 mM MOPS

37°C

Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in µM)

Activity - Michaelis-Menten

Km (in µM) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15 % v/v

2.22

7.45

µM

Direct

Continuous

100 mM MOPS

37°C

Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in µM)

Activity - Michaelis-Menten

Vmax (in U/mg) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

1 % v/v

28019.0

28223.0

U/mg

Direct

Continuous

100 mM MOPS

37°C

Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in U/mg)

Activity - Michaelis-Menten

Vmax (in U/mg) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

3 % v/v

28019.0

31913.0

U/mg

Direct

Continuous

100 mM MOPS

37°C

Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in U/mg)

Activity - Michaelis-Menten

Vmax (in U/mg) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

5 % v/v

28019.0

37400.0

U/mg

Direct

Continuous

100 mM MOPS

37°C

Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in U/mg)

Activity - Michaelis-Menten

Vmax (in U/mg) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

28019.0

45327.0

U/mg

Direct

Continuous

100 mM MOPS

37°C

Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in U/mg)

Activity - Michaelis-Menten

Vmax (in U/mg) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15 % v/v

28019.0

38839.0

U/mg

Direct

Continuous

100 mM MOPS

37°C

Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in U/mg)

Activity - Michaelis-Menten

Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

1 % v/v

12621.0

8301.0

L/(min * mg)

Direct

Continuous

100 mM MOPS

37°C

Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in L/(min * mg))

Activity - Michaelis-Menten

Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

3 % v/v

12621.0

7456.0

L/(min * mg)

Direct

Continuous

100 mM MOPS

37°C

Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in L/(min * mg))

Activity - Michaelis-Menten

Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

5 % v/v

12621.0

9064.0

L/(min * mg)

Direct

Continuous

100 mM MOPS

37°C

Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in L/(min * mg))

Activity - Michaelis-Menten

Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

12621.0

6218.0

L/(min * mg)

Direct

Continuous

100 mM MOPS

37°C

Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in L/(min * mg))

Activity - Michaelis-Menten

Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15 % v/v

12621.0

5213.0

L/(min * mg)

Direct

Continuous

100 mM MOPS

37°C

Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in L/(min * mg))

Activity - Michaelis-Menten

Km (in µM) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

1 % v/v

5.61

5.74

µM

Direct

Continuous

100 mM MOPS

37°C

Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in µM)

Activity - Michaelis-Menten

Km (in µM) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

3 % v/v

5.61

6.81

µM

Direct

Continuous

100 mM MOPS

37°C

Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in µM)

Activity - Michaelis-Menten

Km (in µM) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

5 % v/v

5.61

15.69

µM

Direct

Continuous

100 mM MOPS

37°C

Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in µM)

Activity - Michaelis-Menten

Km (in µM) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

5.61

33.2

µM

Direct

Continuous

100 mM MOPS

37°C

Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in µM)

Activity - Michaelis-Menten

Km (in µM) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15 % v/v

5.61

43.49

µM

Direct

Continuous

100 mM MOPS

37°C

Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in µM)

Activity - Michaelis-Menten

Vmax (in U/mg) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

1 % v/v

19315.0

19513.0

U/mg

Direct

Continuous

100 mM MOPS

37°C

Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in U/mg)

Activity - Michaelis-Menten

Vmax (in U/mg) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

3 % v/v

19315.0

23445.0

U/mg

Direct

Continuous

100 mM MOPS

37°C

Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in U/mg)

Activity - Michaelis-Menten

Vmax (in U/mg) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

5 % v/v

19315.0

42835.0

U/mg

Direct

Continuous

100 mM MOPS

37°C

Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in U/mg)

Activity - Michaelis-Menten

Vmax (in U/mg) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

19315.0

55605.0

U/mg

Direct

Continuous

100 mM MOPS

37°C

Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in U/mg)

Activity - Michaelis-Menten

Vmax (in U/mg) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15 % v/v

19315.0

54254.0

U/mg

Direct

Continuous

100 mM MOPS

37°C

Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in U/mg)

Activity - Michaelis-Menten

Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

1 % v/v

3443.0

3399.0

L/(min * mg)

Direct

Continuous

100 mM MOPS

37°C

Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in L/(min * mg))

Activity - Michaelis-Menten

Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

3 % v/v

3443.0

L/(min * mg)

Direct

Continuous

100 mM MOPS

37°C

Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in L/(min * mg))

Activity - Michaelis-Menten

Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

5 % v/v

3443.0

2730.0

L/(min * mg)

Direct

Continuous

100 mM MOPS

37°C

Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in L/(min * mg))

Activity - Michaelis-Menten

Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

3443.0

1675.0

L/(min * mg)

Direct

Continuous

100 mM MOPS

37°C

Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in L/(min * mg))

Activity - Michaelis-Menten

Vmax/Km (in L/(min*mg)) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15 % v/v

3443.0

1248.0

L/(min * mg)

Direct

Continuous

100 mM MOPS

37°C

Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in L/(min * mg))

Activity + Stability - Incubation

Activity after incubation (96 hours at 37°C) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25 % v/v

1168.0

795.0

U/mg

Direct

Continuous

Incubation: 100 mM MOPS, Assay: 100 mM MOPS

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Non-incubated control (in U/mg), assay in the presence of organic solvent | Clear about assay in the presence of organic solvent and control in aqueous phase

Activity + Stability - Incubation

Activity after incubation (96 hours at 37°C) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

25 % v/v

1168.0

1036.0

U/mg

Direct

Continuous

Incubation: 100 mM MOPS, Assay: 100 mM MOPS

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Non-incubated control (in U/mg), assay in the presence of organic solvent | Clear about assay in the presence of organic solvent and control in aqueous phase

Activity + Stability - Incubation

Activity after incubation (96 hours at 37°C) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25 % v/v

1223.0

1112.0

U/mg

Direct

Continuous

Incubation: 100 mM MOPS, Assay: 100 mM MOPS

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Non-incubated control (in U/mg), assay in the presence of organic solvent | Clear about assay in the presence of organic solvent and control in aqueous phase

Activity + Stability - Incubation

Activity after incubation (96 hours at 37°C) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

25 % v/v

1223.0

1074.0

U/mg

Direct

Continuous

Incubation: 100 mM MOPS, Assay: 100 mM MOPS

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Non-incubated control (in U/mg), assay in the presence of organic solvent | Clear about assay in the presence of organic solvent and control in aqueous phase

Stability - C50

Concentration (in % v/v) for which 50% of enzyme activity is lost, as measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

—

33.0

% (v/v)

Direct

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Not applicable control (in % (v/v))

Stability - C50

Concentration (in % v/v) for which 50% of enzyme activity is lost, as measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

—

23.0

% (v/v)

Direct

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Not applicable control (in % (v/v))

Stability - C50

Concentration (in % v/v) for which 50% of enzyme activity is lost, as measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

—

19.0

% (v/v)

Direct

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Not applicable control (in % (v/v))

Stability - C50

Concentration (in % v/v) for which 50% of enzyme activity is lost, as measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

—

8.0

% (v/v)

Direct

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Not applicable control (in % (v/v))

Stability - C50

Concentration (in % v/v) for which 50% of enzyme activity is lost, as measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1,4-Dioxane

—

13.0

% (v/v)

Direct

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Not applicable control (in % (v/v))

Stability - Incubation

Activity after incubation (96 hours at 37°C) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25 % v/v

1187.0

795.0

U/mg

Direct

Continuous

Incubation: 100 mM MOPS, Assay: 100 mM MOPS

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Non-incubated control (in U/mg) in the presence of organic solvent (1 minute incubation, 5% DMSO), assay in the presence of organic solvent | Clear about assay in the presence of organic solvent and control in the presence of organic solvent

Stability - Incubation

Activity after incubation (96 hours at 37°C) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

25 % v/v

1276.0

1036.0

U/mg

Direct

Continuous

Incubation: 100 mM MOPS, Assay: 100 mM MOPS

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Non-incubated control (in U/mg) in the presence of organic solvent (1 minute incubation, 5% acetone), assay in the presence of organic solvent | Clear about assay in the presence of organic solvent and control in the presence of organic solvent

Stability - Incubation

Activity after incubation (96 hours at 37°C) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25 % v/v

1178.0

1112.0

U/mg

Direct

Continuous

Incubation: 100 mM MOPS, Assay: 100 mM MOPS

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Stability - Incubation

Activity after incubation (96 hours at 37°C) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

25 % v/v

952.0

1074.0

U/mg

Direct

Continuous

Incubation: 100 mM MOPS, Assay: 100 mM MOPS

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity — Michaelis-Menten

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity + Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — C₅₀

One bar per measurement. Colour = solvent, shade = temperature. Hover for details.

Structure

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Feruloyl esterase C (gene faeC) from Talaromyces stipitatus

Enzyme Description

Sequence

Experimental Data (133 measurements)

Visualization : Activity — Classical

Visualization : Activity — Michaelis-Menten

Visualization : Stability — Incubation

Visualization : Activity + Stability — Incubation

Visualization : Stability — C50

Structure

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Visualization : Stability — C₅₀