Ene-reductase KYE1 from Kluyveromyces lactis

Enzyme Description

Species
Kluyveromyces lactis β†—
Extremophile
No
EC Number
1.3.1.31 β†—

Sequence

Length: 398 amino acids
MSFMNFEPKPLADTDIFKPIKIGNTELKHRVVMPALTRMRALHPGNVPNPDWAVEYYRQRSQYPGTMIITEGAFPSAQSGGYDNAPGVWSEEQLAQWRKIFKAIHDNKSFVWVQLWVLGRQAFADNLARDGLRYDSASDEVYMGEDEKERAIRSNNPQHGITKDEIKQYIRDYVDAAKKCIDAGADGVEIHSANGYLLNQFLDPISNKRTDEYGGSIENRARFVLEVVDAVVDAVGAERTSIRFSPYGVFGTMSGGSDPVLVAQFAYVLAELEKRAKAGKRLAYVDLVEPRVTSPFQPEFEGWYKGGTNEFVYSVWKGNVLRVGNYALDPDAAITDSKNPNTLIGYGRAFIANPDLVERLEKGLPLNQYDRPSFYKMSAEGYIDYPTYEEAVAKGYKK
Yanto Yanto et al. (2011) β€” Asymmetric bioreduction of alkenes using ene-reductases YersER and KYE1 and effects of organic solvents
Organic Letters  Β· doi:10.1021/ol200394p β†—  Β· Stability - C50 Activity + Stability - Conversion Selectivity - Enantioselectivity
84 measurements ▼
Database ID
UniProt: P40952 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21

Experimental Data (84 measurements)

84 measurements β€” page 1 of 5
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Ethylene Glycol 50 % v/v 67.0 35.0 % Digital Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM Citral Citronellal 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Methanol 20 % v/v 100.0 23.7 % Direct Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM 2-Cyclohexen-1-one Cyclohexanone 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Propylene Glycol 20 % v/v 100.0 3.2 % Direct Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM 2-Cyclohexen-1-one Cyclohexanone 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Ethanol 20 % v/v 100.0 6.6 % Direct Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM 2-Cyclohexen-1-one Cyclohexanone 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Dimethyl Sulfoxide (DMSO) 20 % v/v 100.0 98.5 % Direct Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM 2-Cyclohexen-1-one Cyclohexanone 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Dimethylformamide (DMF) 20 % v/v 100.0 4.1 % Direct Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM 2-Cyclohexen-1-one Cyclohexanone 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Acetonitrile 20 % v/v 100.0 0.2 % Direct Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM 2-Cyclohexen-1-one Cyclohexanone 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Acetone 20 % v/v 100.0 5.3 % Direct Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM 2-Cyclohexen-1-one Cyclohexanone 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography 1,4-Dioxane 20 % v/v 100.0 9.1 % Direct Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM 2-Cyclohexen-1-one Cyclohexanone 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Tetrahydrofuran (THF) 20 % v/v 100.0 0.7 % Direct Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM 2-Cyclohexen-1-one Cyclohexanone 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Toluene 20 % v/v 100.0 97.8 % Direct Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM 2-Cyclohexen-1-one Cyclohexanone 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography n-Hexane 20 % v/v 100.0 95.3 % Direct Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM 2-Cyclohexen-1-one Cyclohexanone 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Ethylene Glycol 10 % v/v 67.0 67.0 % Digital Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM Citral Citronellal 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Ethylene Glycol 20 % v/v 67.0 61.0 % Digital Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM Citral Citronellal 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Ethylene Glycol 30 % v/v 67.0 62.0 % Digital Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM Citral Citronellal 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Ethylene Glycol 40 % v/v 67.0 50.0 % Digital Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM Citral Citronellal 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Ethylene Glycol 20 % v/v 100.0 100.0 % Digital Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM 2-Cyclohexen-1-one Cyclohexanone 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Dimethyl Sulfoxide (DMSO) 10 % v/v 67.0 70.0 % Digital Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM Citral Citronellal 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Dimethyl Sulfoxide (DMSO) 20 % v/v 67.0 51.0 % Digital Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM Citral Citronellal 0.2 mM NADP+ β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (12 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography Dimethyl Sulfoxide (DMSO) 30 % v/v 67.0 21.0 % Digital Continuous 200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling) 7.5 30Β°C 10 mM Citral Citronellal 0.2 mM NADP+ β€” Classical aqueous control (in %)
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Visualization : Stability β€” C50

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Visualization : Activity + Stability β€” Conversion

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Visualization : Selectivity - Enantioselectivity

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Structure

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