Haloalkane dehalogenase linB from Sphingobium japonicum

Enzyme Description

Species

Extremophile

EC Number

3.8.1.5 ↗

Sequence

Length: 296 amino acids

MSLGAKPFGEKKFIEIKGRRMAYIDEGTGDPILFQHGNPTSSYLWRNIMPHCAGLGRLIACDLIGMGDSDKLDPSGPERYAYAEHRDYLDALWEALDLGDRVVLVVHDWGSALGFDWARRHRERVQGIAYMEAIAMPIEWADFPEQDRDLFQAFRSQAGEELVLQDNVFVEQVLPGLILRPLSEAEMAAYREPFLAAGEARRPTLSWPRQIPIAGTPADVVAIARDYAGWLSESPIPKLFINAEPGALTTGRMRDFCRTWPNQTEITVAGAHFIQEDSPDEIGAAIAAFVRRLRPA

Veronika Stepankova et al. (2013) — Organic co-solvents affect activity, stability and enantioselectivity of haloalkane dehalogenases

Biotechnology Journal · doi:10.1002/biot.201200378 ↗ · Activity - Classical Stability - C50 Stability - Tm

136 measurements ▼

Database ID

UniProt: D4Z2G1 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Recombinant, host bacterium Escherichia coli BL21

Experimental Data (136 measurements)

136 measurements — page 1 of 7

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	Glycerol	5 % v/v	100	91	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	Formamide	5 % v/v	100	88	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	Ethylene Glycol	5 % v/v	100	92	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	PEG-1000	5 % v/v	100	101	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	PEG-10000	5 % v/v	100	85	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	Dimethylformamide (DMF)	5 % v/v	100	81	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5 % v/v	100	86	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	Methanol	5 % v/v	100	93	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	1,4-Dioxane	5 % v/v	100	41	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	Acetonitrile	5 % v/v	100	71	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	Ethanol	5 % v/v	100	93	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	Acetone	5 % v/v	100	110	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	Isopropanol	5 % v/v	100	85	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	5 % v/v	100	64	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	Glycerol	10 % v/v	100	90	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	Formamide	10 % v/v	100	80	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	Ethylene Glycol	10 % v/v	100	101	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	PEG-1000	10 % v/v	100	104	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	PEG-10000	10 % v/v	100	80	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10 % v/v	100	78	%	Direct	Continuous	glycine 100 mM	8.6	37°C	? mM 1-Iodohexane	1-Hexanol , Iodide ion	—	—	Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent

Glycerol

5 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Formamide

5 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Ethylene Glycol

5 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

PEG-1000

5 % v/v

100

101

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

PEG-10000

5 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Dimethylformamide (DMF)

5 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Dimethyl Sulfoxide (DMSO)

5 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Methanol

5 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

1,4-Dioxane

5 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Acetonitrile

5 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Ethanol

5 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Acetone

5 % v/v

100

110

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Isopropanol

5 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Tetrahydrofuran (THF)

5 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Glycerol

10 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Formamide

10 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Ethylene Glycol

10 % v/v

100

101

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

PEG-1000

10 % v/v

100

104

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

PEG-10000

10 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Dimethylformamide (DMF)

10 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Methanol

10 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

1,4-Dioxane

10 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Acetonitrile

10 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Ethanol

10 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Acetone

10 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Isopropanol

10 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Tetrahydrofuran (THF)

10 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Glycerol

20 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Formamide

20 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Ethylene Glycol

20 % v/v

100

109

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

PEG-1000

20 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

PEG-10000

20 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Dimethylformamide (DMF)

20 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Dimethyl Sulfoxide (DMSO)

20 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Methanol

20 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

1,4-Dioxane

20 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Acetonitrile

20 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Ethanol

20 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Acetone

20 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Isopropanol

20 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Tetrahydrofuran (THF)

20 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Glycerol

30 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Formamide

30 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Ethylene Glycol

30 % v/v

100

118

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

PEG-1000

30 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

PEG-10000

30 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Dimethylformamide (DMF)

30 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Dimethyl Sulfoxide (DMSO)

30 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Methanol

30 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

1,4-Dioxane

30 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Acetonitrile

30 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Ethanol

30 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Acetone

30 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Isopropanol

30 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Tetrahydrofuran (THF)

30 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Glycerol

40 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Formamide

40 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Ethylene Glycol

40 % v/v

100

122

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

PEG-1000

40 % v/v

100

102

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

PEG-10000

40 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Dimethylformamide (DMF)

40 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Dimethyl Sulfoxide (DMSO)

40 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Methanol

40 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

1,4-Dioxane

40 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Acetonitrile

40 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Ethanol

40 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Acetone

40 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Isopropanol

40 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Tetrahydrofuran (THF)

40 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Glycerol

50 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Formamide

50 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Ethylene Glycol

50 % v/v

100

119

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

PEG-1000

50 % v/v

100

101

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

PEG-10000

50 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Dimethylformamide (DMF)

50 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Dimethyl Sulfoxide (DMSO)

50 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Methanol

50 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

1,4-Dioxane

50 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Acetonitrile

50 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Ethanol

50 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Acetone

50 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Isopropanol

50 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Tetrahydrofuran (THF)

50 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Glycerol

60 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Formamide

60 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Ethylene Glycol

60 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

PEG-1000

60 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

PEG-10000

60 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Dimethylformamide (DMF)

60 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Dimethyl Sulfoxide (DMSO)

60 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Methanol

60 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

1,4-Dioxane

60 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Acetonitrile

60 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Ethanol

60 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Acetone

60 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Isopropanol

60 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Tetrahydrofuran (THF)

60 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Glycerol

75 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Formamide

75 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Ethylene Glycol

75 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

PEG-1000

75 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

PEG-10000

75 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Dimethylformamide (DMF)

75 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Dimethyl Sulfoxide (DMSO)

75 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Methanol

75 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

1,4-Dioxane

75 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Acetonitrile

75 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Ethanol

75 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Acetone

75 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Isopropanol

75 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Activity - Classical

Tetrahydrofuran (THF)

75 % v/v

100

Direct

Continuous

glycine 100 mM

8.6

37°C

? mM 1-Iodohexane

1-Hexanol, Iodide ion

—

Classical aqueous control (in %)

Stability - C50

Volume fraction of organic solvent at which 50% of enzyme activity is lost, measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product Fe–SCN absorbance measurement, 460 nm) in the presence of organic solvent

Glycerol

—

% (v/v)

Direct

Continuous

glycine 100 mM

8.6

37°C

1-Iodohexane

1-Hexanol, Iodide ion

—

Not applicable control (in % (v/v))

Stability - C50

Formamide

—

% (v/v)

Direct

Continuous

glycine 100 mM

8.6

37°C

1-Iodohexane

1-Hexanol, Iodide ion

—

Not applicable control (in % (v/v))

Stability - C50

Ethylene Glycol

—

% (v/v)

Direct

Continuous

glycine 100 mM

8.6

37°C

1-Iodohexane

1-Hexanol, Iodide ion

—

Not applicable control (in % (v/v))

Stability - C50

Dimethyl Sulfoxide (DMSO)

—

% (v/v)

Direct

Continuous

glycine 100 mM

8.6

37°C

1-Iodohexane

1-Hexanol, Iodide ion

—

Not applicable control (in % (v/v))

Stability - C50

Dimethylformamide (DMF)

—

% (v/v)

Direct

Continuous

glycine 100 mM

8.6

37°C

1-Iodohexane

1-Hexanol, Iodide ion

—

Not applicable control (in % (v/v))

Stability - C50

Methanol

—

% (v/v)

Direct

Continuous

glycine 100 mM

8.6

37°C

1-Iodohexane

1-Hexanol, Iodide ion

—

Not applicable control (in % (v/v))

Stability - C50

Acetonitrile

—

% (v/v)

Direct

Continuous

glycine 100 mM

8.6

37°C

1-Iodohexane

1-Hexanol, Iodide ion

—

Not applicable control (in % (v/v))

Stability - C50

Ethanol

—

% (v/v)

Direct

Continuous

glycine 100 mM

8.6

37°C

1-Iodohexane

1-Hexanol, Iodide ion

—

Not applicable control (in % (v/v))

Stability - C50

1,4-Dioxane

—

% (v/v)

Direct

Continuous

glycine 100 mM

8.6

37°C

1-Iodohexane

1-Hexanol, Iodide ion

—

Not applicable control (in % (v/v))

Stability - C50

Acetone

—

% (v/v)

Direct

Continuous

glycine 100 mM

8.6

37°C

1-Iodohexane

1-Hexanol, Iodide ion

—

Not applicable control (in % (v/v))

Stability - C50

Isopropanol

—

% (v/v)

Direct

Continuous

glycine 100 mM

8.6

37°C

1-Iodohexane

1-Hexanol, Iodide ion

—

Not applicable control (in % (v/v))

Stability - C50

Tetrahydrofuran (THF)

—

% (v/v)

Direct

Continuous

glycine 100 mM

8.6

37°C

1-Iodohexane

1-Hexanol, Iodide ion

—

Not applicable control (in % (v/v))

Stability - Tm

Tm measured by circular dichroism in the presence of organic solvent

Ethylene Glycol

20 % v/v

°C

Direct

Continuous

50 mM phosphate buffer

7.5

—

Classical aqueous control (in °C), measured by circular dichroism

Stability - Tm

Tm measured by DSC in the presence of organic solvent

Dimethylformamide (DMF)

20 % v/v

°C

Direct

Continuous

50 mM phosphate buffer

7.5

—

Classical aqueous control (in °C) measured by DSC

Stability - Tm

Tm measured by DSC in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

°C

Direct

Continuous

50 mM phosphate buffer

7.5

—

Classical aqueous control (in °C) measured by DSC

Stability - Tm

Tm measured by circular dichroism in the presence of organic solvent

Methanol

20 % v/v

°C

Direct

Continuous

50 mM phosphate buffer

7.5

—

Classical aqueous control (in °C), measured by circular dichroism

Stability - Tm

Tm measured by circular dichroism in the presence of organic solvent

1,4-Dioxane

20 % v/v

°C

Direct

Continuous

50 mM phosphate buffer

7.5

—

Classical aqueous control (in °C), measured by circular dichroism

Stability - Tm

Tm measured by circular dichroism in the presence of organic solvent

Acetonitrile

10 % v/v

°C

Direct

Continuous

50 mM phosphate buffer

7.5

—

Classical aqueous control (in °C), measured by circular dichroism

Stability - Tm

Tm measured by circular dichroism in the presence of organic solvent

Acetonitrile

20 % v/v

<10

°C

Direct

Continuous

50 mM phosphate buffer

7.5

—

Classical aqueous control (in °C), measured by circular dichroism

Stability - Tm

Tm measured by circular dichroism in the presence of organic solvent

Ethanol

20 % v/v

°C

Direct

Continuous

50 mM phosphate buffer

7.5

—

Classical aqueous control (in °C), measured by circular dichroism

Stability - Tm

Tm measured by circular dichroism in the presence of organic solvent

Acetone

20 % v/v

°C

Direct

Continuous

50 mM phosphate buffer

7.5

—

Classical aqueous control (in °C), measured by circular dichroism

Stability - Tm

Tm measured by circular dichroism in the presence of organic solvent

Isopropanol

20 % v/v

°C

Direct

Continuous

50 mM phosphate buffer

7.5

—

Classical aqueous control (in °C), measured by circular dichroism

Stability - Tm

Tm measured by circular dichroism in the presence of organic solvent

Tetrahydrofuran (THF)

10 % v/v

°C

Direct

Continuous

50 mM phosphate buffer

7.5

—

Classical aqueous control (in °C), measured by circular dichroism

Stability - Tm

Tm measured by circular dichroism in the presence of organic solvent

Tetrahydrofuran (THF)

20 % v/v

<10

°C

Direct

Continuous

50 mM phosphate buffer

7.5

—

Classical aqueous control (in °C), measured by circular dichroism

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — T_m

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — C₅₀

One bar per measurement. Colour = solvent, shade = temperature. Hover for details.

Structure

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Haloalkane dehalogenase linB from Sphingobium japonicum

Enzyme Description

Sequence

Experimental Data (136 measurements)

Visualization : Activity — Classical

Visualization : Stability — Tm

Visualization : Stability — C50

Structure

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Visualization : Stability — T_m

Visualization : Stability — C₅₀