Aldehyde oxidase 1 from Oryctolagus cuniculus (rabbit)

Enzyme Description

Species
Oryctolagus cuniculus (rabbit) β†—
Extremophile
No
EC Number
1.2.3.1 β†—

Sequence

Length: 1334 amino acids
MEPAPELLFYVNGRKVVEKQVDPETMLLPYLRKKLRLTGTKYGCGGGGCGACTVMISRYNRVTKKIRHYPVNACLTPICSLYGAAVTTVEGIGSTTTRLHPVQERIAKFHGTQCGFCTPGMVMSMYALLRNHPEPTLDQLADALGGNLCRCTGYRPIIEAYKTFCKTSDCCQNKENGFCCLDQGINGLPEVEEENQTRPNLFSEEEYLPLDPTQELIFPPELMTMAEKQPQRTRVFSGERMMWISPVTLKALLEAKSTYPQAPVVMGNTSVGPGVKFKGIFHPVIISPDSIEELNVVSHTHSGLTLGAGLSLAQVKDILADVVQKVPEENAQTYRALLKHLGTLAGSQIRNMASLGGHIISRHLDSDLNPLLAVGNCTLNVLSKEGERQIPLDEQFLSRCPEADLKPQEILASVHIPYSRKWEFVLAFRQAQRKQNALAIVNSGMRVFFGEGDGIIRELAISYGGVGPTIICAKNSCQKLIGRSWNEEMLDTACRLILDEVSLPGSAPGGKVEFKRTLIISFLFKFYLEVSQILKRMAPGLSPHLADKYESALQDLHARYSWSTLKDQDVDARQLSQDPIGHPVMHLSGVKHATGEAIYLDDMPAVDQELFMAFVTSPRAHAKIVSTDLLEALSLPGVVDIVTAEHLQDGNTFYTEKLLAADEVLCVGQLVCAVIAESEVQAKQAAKQVKIVYEDLEPVILSIEEAIEQKSFFEPERKLEYGNVDEAFKVVDQILEGEIHMGGQEHFYMETQSVLVVPKGEDQEMDVYASTQFPKYIQDMVAAVLKLPVNKVMCHVKRVGGAFGGKVFKASIMAAIAAFAANKHGRAVRCILERGEDMLITGGRHPYLGKYKAGFMNDGRIVALDVEHYSNGGCSLDESLLVIEMGLLKMENAYKFPNLRCRGWACRTNLPSNTAFRGFGFPQAGLITECCITEVAAKCGLSPEKVRAINFYKEIDQTPYKQEINAKNLTQCWNECLAKSSYFQRKVAVEKFNAENYWKQRGLAIIPFKYPRGLGSVAYGQAAALVHVYLDGSVLVTHGGIEMGQGVHTKMIQVVSRELKMPMSNVHLRGTSTETVPNTNASGGSVVADLNGLAVKDACQTLLKRLEPIINKNPQGTWKEWAQAAFDKSISLSATGYFRGYDSNIDWDKGEGHPFEYFVYGAACSEVEIDCLTGDHKTIRTDIVMDVGYSINPALDIGQVEGAFIQGMGLYTIEELHYSPQGILYSRGPNQYKIPAICDIPAELNVTFLPPSEKSNTLYSSKGLGESGVFMGCSVFFAIREAVCAARQARGLSAPWKLSSPLTPEKIRMACEDKFTKMIPRDKPGSYVPWNVPV
Mohammad-Reza Rashidi et al. (2013) β€” Aldehyde Oxidase Activity and Stability in Water-Miscible Organic Solvents
Applied Biochemistry and Biotechnology  Β· doi:10.1007/s12010-012-0036-7 β†—  Β· Activity - Classical Activity - Michaelis-Menten Stability - C50 Stability - Incubation
243 measurements ▼
Database ID
UniProt: P80456 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, eukaryote Oryctolagus cuniculus (rabbit)

Experimental Data (243 measurements)

243 measurements β€” page 11 of 13
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Tetrahydrofuran (THF) β€” β€” 0.5 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 45Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Dimethylformamide (DMF) β€” β€” 20.0 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 25Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Dimethylformamide (DMF) β€” β€” 13.0 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Dimethylformamide (DMF) β€” β€” 12.0 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 45Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent 1-Propanol β€” β€” 2.0 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 25Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent 1-Propanol β€” β€” 2.0 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent 1-Propanol β€” β€” 1.5 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 45Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Isopropanol β€” β€” 5.0 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 25Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Isopropanol β€” β€” 5.0 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Isopropanol β€” β€” 4.0 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 45Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent 1,4-Dioxane β€” β€” 10.0 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 25Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent 1,4-Dioxane β€” β€” 9.0 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent 1,4-Dioxane β€” β€” 8.0 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 45Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Pyridine β€” β€” 0.5 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 25Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Pyridine β€” β€” 0.5 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Pyridine β€” β€” 0.5 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 45Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Methanol β€” β€” 0.5 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 25Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Methanol β€” β€” 0.5 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 35Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Methanol β€” β€” 1.5 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 45Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent Acetonitrile β€” β€” 5.0 % (v/v) Digital Continuous 67 mM Sorenson phosphate buffer, 0.1 MM EDTA 7.4 25Β°C 20 Β΅M Phenanthridine Phenanthridinone β€” β€” Not applicable control (in % (v/v))
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Visualization : Activity β€” Classical

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Visualization : Activity β€” Michaelis-Menten

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Visualization : Stability β€” Incubation

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Visualization : Stability β€” C50

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Structure

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