Aldehyde oxidase 1 from Oryctolagus cuniculus (rabbit)

Enzyme Description

Species

Extremophile

EC Number

1.2.3.1 ↗

Sequence

Length: 1334 amino acids

MEPAPELLFYVNGRKVVEKQVDPETMLLPYLRKKLRLTGTKYGCGGGGCGACTVMISRYNRVTKKIRHYPVNACLTPICSLYGAAVTTVEGIGSTTTRLHPVQERIAKFHGTQCGFCTPGMVMSMYALLRNHPEPTLDQLADALGGNLCRCTGYRPIIEAYKTFCKTSDCCQNKENGFCCLDQGINGLPEVEEENQTRPNLFSEEEYLPLDPTQELIFPPELMTMAEKQPQRTRVFSGERMMWISPVTLKALLEAKSTYPQAPVVMGNTSVGPGVKFKGIFHPVIISPDSIEELNVVSHTHSGLTLGAGLSLAQVKDILADVVQKVPEENAQTYRALLKHLGTLAGSQIRNMASLGGHIISRHLDSDLNPLLAVGNCTLNVLSKEGERQIPLDEQFLSRCPEADLKPQEILASVHIPYSRKWEFVLAFRQAQRKQNALAIVNSGMRVFFGEGDGIIRELAISYGGVGPTIICAKNSCQKLIGRSWNEEMLDTACRLILDEVSLPGSAPGGKVEFKRTLIISFLFKFYLEVSQILKRMAPGLSPHLADKYESALQDLHARYSWSTLKDQDVDARQLSQDPIGHPVMHLSGVKHATGEAIYLDDMPAVDQELFMAFVTSPRAHAKIVSTDLLEALSLPGVVDIVTAEHLQDGNTFYTEKLLAADEVLCVGQLVCAVIAESEVQAKQAAKQVKIVYEDLEPVILSIEEAIEQKSFFEPERKLEYGNVDEAFKVVDQILEGEIHMGGQEHFYMETQSVLVVPKGEDQEMDVYASTQFPKYIQDMVAAVLKLPVNKVMCHVKRVGGAFGGKVFKASIMAAIAAFAANKHGRAVRCILERGEDMLITGGRHPYLGKYKAGFMNDGRIVALDVEHYSNGGCSLDESLLVIEMGLLKMENAYKFPNLRCRGWACRTNLPSNTAFRGFGFPQAGLITECCITEVAAKCGLSPEKVRAINFYKEIDQTPYKQEINAKNLTQCWNECLAKSSYFQRKVAVEKFNAENYWKQRGLAIIPFKYPRGLGSVAYGQAAALVHVYLDGSVLVTHGGIEMGQGVHTKMIQVVSRELKMPMSNVHLRGTSTETVPNTNASGGSVVADLNGLAVKDACQTLLKRLEPIINKNPQGTWKEWAQAAFDKSISLSATGYFRGYDSNIDWDKGEGHPFEYFVYGAACSEVEIDCLTGDHKTIRTDIVMDVGYSINPALDIGQVEGAFIQGMGLYTIEELHYSPQGILYSRGPNQYKIPAICDIPAELNVTFLPPSEKSNTLYSSKGLGESGVFMGCSVFFAIREAVCAARQARGLSAPWKLSSPLTPEKIRMACEDKFTKMIPRDKPGSYVPWNVPV

Mohammad-Reza Rashidi et al. (2013) — Aldehyde Oxidase Activity and Stability in Water-Miscible Organic Solvents

Applied Biochemistry and Biotechnology · doi:10.1007/s12010-012-0036-7 ↗ · Activity - Classical Activity - Michaelis-Menten Stability - C50 Stability - Incubation

243 measurements ▼

Database ID

UniProt: P80456 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, eukaryote Oryctolagus cuniculus (rabbit)

Experimental Data (243 measurements)

243 measurements — page 12 of 13

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	—	—	10.0	% (v/v)	Digital	Continuous	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	—	—	15.0	% (v/v)	Digital	Continuous	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	—	—	17.0	% (v/v)	Digital	Continuous	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	—	—	15.0	% (v/v)	Digital	Continuous	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	—	—	10.0	% (v/v)	Digital	Continuous	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Pyridine	0.5 % v/v	98.0	0.0	%	Digital	Continuous	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	1,4-Dioxane	6 % v/v	98.0	21.0	%	Digital	Continuous	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Tetrahydrofuran (THF)	0.5 % v/v	98.0	43.0	%	Digital	Continuous	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Ethanol	5 % v/v	98.0	46.0	%	Digital	Continuous	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	1-Propanol	1 % v/v	98.0	56.0	%	Digital	Continuous	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Isopropanol	2 % v/v	98.0	60.0	%	Digital	Continuous	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Dimethylformamide (DMF)	6 % v/v	98.0	63.0	%	Digital	Continuous	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Methanol	0.5 % v/v	98.0	63.0	%	Digital	Continuous	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Acetonitrile	5 % v/v	98.0	63.0	%	Digital	Continuous	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Pyridine	0.5 % v/v	100.0	0.0	%	Digital	Continuous	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Ethanol	5 % v/v	100.0	7.0	%	Digital	Continuous	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Dimethylformamide (DMF)	6 % v/v	100.0	8.0	%	Digital	Continuous	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Isopropanol	2 % v/v	100.0	12.0	%	Digital	Continuous	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Tetrahydrofuran (THF)	0.5 % v/v	100.0	13.0	%	Digital	Continuous	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	1-Propanol	1 % v/v	100.0	20.0	%	Digital	Continuous	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5 % v/v

100.0

56.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

1 % v/v

100.0

42.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

1.5 % v/v

100.0

35.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

2 % v/v

100.0

30.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

2.5 % v/v

100.0

23.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

5 % v/v

100.0

10.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

10 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

15 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

20 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

25 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

30 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

35 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

0.5 % v/v

100.0

98.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

1 % v/v

100.0

94.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

1.5 % v/v

100.0

90.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

2 % v/v

100.0

87.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

2.5 % v/v

100.0

82.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

5 % v/v

100.0

73.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

10 % v/v

100.0

63.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

15 % v/v

100.0

48.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

20 % v/v

100.0

39.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

25 % v/v

100.0

25.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

30 % v/v

100.0

10.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

35 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

0.5 % v/v

100.0

69.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

1 % v/v

100.0

60.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

1.5 % v/v

100.0

54.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

2 % v/v

100.0

49.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

2.5 % v/v

100.0

40.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

5 % v/v

100.0

30.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

10 % v/v

100.0

17.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

15 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

20 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

25 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

30 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

35 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

0.5 % v/v

100.0

80.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

1 % v/v

100.0

74.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

1.5 % v/v

100.0

70.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

2 % v/v

100.0

65.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

2.5 % v/v

100.0

56.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

5 % v/v

100.0

43.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

10 % v/v

100.0

26.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

15 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

20 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

25 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

30 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

35 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

0.5 % v/v

100.0

95.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

1 % v/v

100.0

90.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

1.5 % v/v

100.0

85.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

2 % v/v

100.0

78.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

2.5 % v/v

100.0

70.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

5 % v/v

100.0

60.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

10 % v/v

100.0

43.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

15 % v/v

100.0

28.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

20 % v/v

100.0

13.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

25 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

30 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

35 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

0.5 % v/v

100.0

40.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

1 % v/v

100.0

22.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

1.5 % v/v

100.0

17.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

2 % v/v

100.0

13.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

2.5 % v/v

100.0

5.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

5 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

10 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

15 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

20 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

25 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

30 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

35 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

0.5 % v/v

100.0

65.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

1 % v/v

100.0

45.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

1.5 % v/v

100.0

38.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

2 % v/v

100.0

32.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

2.5 % v/v

100.0

26.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

5 % v/v

100.0

15.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

10 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

15 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

20 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

25 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

30 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

35 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

0.5 % v/v

100.0

95.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

1 % v/v

100.0

88.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

1.5 % v/v

100.0

83.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

2 % v/v

100.0

79.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

2.5 % v/v

100.0

72.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

5 % v/v

100.0

63.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

10 % v/v

100.0

41.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

15 % v/v

100.0

17.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

20 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

25 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

30 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

35 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

0.5 % v/v

100.0

98.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

1 % v/v

100.0

93.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

1.5 % v/v

100.0

88.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

2 % v/v

100.0

83.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

2.5 % v/v

100.0

77.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

5 % v/v

100.0

68.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

10 % v/v

100.0

55.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

15 % v/v

100.0

42.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

20 % v/v

100.0

33.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

25 % v/v

100.0

20.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

30 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

35 % v/v

100.0

0.0

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5 % v/v

57.0

28.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5 % v/v

71.0

49.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5 % v/v

94.0

65.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

6 % v/v

57.0

37.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

6 % v/v

71.0

50.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

6 % v/v

94.0

73.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

1 % v/v

57.0

21.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

1 % v/v

71.0

37.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

1 % v/v

94.0

57.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

2 % v/v

57.0

22.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

2 % v/v

71.0

34.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

2 % v/v

94.0

73.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

6 % v/v

57.0

20.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

6 % v/v

71.0

28.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

6 % v/v

94.0

33.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

0.5 % v/v

57.0

13.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

0.5 % v/v

71.0

25.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

0.5 % v/v

94.0

33.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

0.5 % v/v

57.0

35.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

0.5 % v/v

71.0

50.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

0.5 % v/v

94.0

82.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

5 % v/v

57.0

21.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

5 % v/v

71.0

48.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

5 % v/v

94.0

67.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

5 % v/v

57.0

30.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

5 % v/v

71.0

45.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

5 % v/v

94.0

67.0

µM/mg product/min

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Tetrahydrofuran (THF)

0.5 % v/v

17.0

10.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Tetrahydrofuran (THF)

0.5 % v/v

50.0

30.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Tetrahydrofuran (THF)

0.5 % v/v

51.0

34.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Tetrahydrofuran (THF)

0.5 % v/v

63.0

36.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Dimethylformamide (DMF)

6 % v/v

17.0

10.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Dimethylformamide (DMF)

6 % v/v

50.0

21.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Dimethylformamide (DMF)

6 % v/v

51.0

39.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Dimethylformamide (DMF)

6 % v/v

63.0

43.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

1-Propanol

1 % v/v

17.0

11.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

1-Propanol

1 % v/v

50.0

28.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

1-Propanol

1 % v/v

51.0

36.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

1-Propanol

1 % v/v

63.0

22.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Isopropanol

2 % v/v

17.0

7.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Isopropanol

2 % v/v

50.0

21.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Isopropanol

2 % v/v

51.0

28.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Isopropanol

2 % v/v

63.0

35.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

1,4-Dioxane

6 % v/v

17.0

10.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

1,4-Dioxane

6 % v/v

50.0

28.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

1,4-Dioxane

6 % v/v

51.0

30.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

1,4-Dioxane

6 % v/v

63.0

28.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Pyridine

0.5 % v/v

17.0

8.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Pyridine

0.5 % v/v

50.0

18.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Pyridine

0.5 % v/v

51.0

27.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Pyridine

0.5 % v/v

63.0

27.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Methanol

0.5 % v/v

17.0

11.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Methanol

0.5 % v/v

50.0

29.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Methanol

0.5 % v/v

51.0

42.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Methanol

0.5 % v/v

63.0

35.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Acetonitrile

5 % v/v

17.0

3.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Acetonitrile

5 % v/v

50.0

32.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Acetonitrile

5 % v/v

51.0

29.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Acetonitrile

5 % v/v

63.0

27.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Ethanol

5 % v/v

17.0

11.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Ethanol

5 % v/v

50.0

26.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Ethanol

5 % v/v

51.0

34.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Classical

Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)

Ethanol

5 % v/v

63.0

28.0

µM/mg product/min

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

35°C

20 µM Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/mg product/min)

Activity - Michaelis-Menten

Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

0.5 % v/v

11.0

5.0

µM

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM), activity measured in aqueous phase

Activity - Michaelis-Menten

Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

2 % v/v

11.0

7.69

µM

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM), activity measured in aqueous phase

Activity - Michaelis-Menten

Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

5 % v/v

11.0

6.3

µM

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM), activity measured in aqueous phase

Activity - Michaelis-Menten

Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

1 % v/v

11.0

8.33

µM

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM), activity measured in aqueous phase

Activity - Michaelis-Menten

Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

6 % v/v

11.0

10.0

µM

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM), activity measured in aqueous phase

Activity - Michaelis-Menten

Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

6 % v/v

11.0

6.66

µM

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM), activity measured in aqueous phase

Activity - Michaelis-Menten

Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

5 % v/v

11.0

8.33

µM

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM), activity measured in aqueous phase

Activity - Michaelis-Menten

Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5 % v/v

11.0

µM

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM), activity measured in aqueous phase

Activity - Michaelis-Menten

Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

0.5 % v/v

11.0

6.25

µM

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM), activity measured in aqueous phase

Activity - Michaelis-Menten

Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

0.5 % v/v

0.039

0.018

µM/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/min), activity measured in aqueous phase

Activity - Michaelis-Menten

Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

2 % v/v

0.039

0.02

µM/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/min), activity measured in aqueous phase

Activity - Michaelis-Menten

Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

5 % v/v

0.039

0.013

µM/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/min), activity measured in aqueous phase

Activity - Michaelis-Menten

Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

1 % v/v

0.039

0.024

µM/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/min), activity measured in aqueous phase

Activity - Michaelis-Menten

Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

6 % v/v

0.039

0.014

µM/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/min), activity measured in aqueous phase

Activity - Michaelis-Menten

Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

6 % v/v

0.039

0.006

µM/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/min), activity measured in aqueous phase

Activity - Michaelis-Menten

Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

5 % v/v

0.039

0.022

µM/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/min), activity measured in aqueous phase

Activity - Michaelis-Menten

Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5 % v/v

0.039

0.025

µM/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/min), activity measured in aqueous phase

Activity - Michaelis-Menten

Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

0.5 % v/v

0.039

0.013

µM/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in µM/min), activity measured in aqueous phase

Activity - Michaelis-Menten

Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Methanol

0.5 % v/v

3.6

1000 * 1/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in 1000 * 1/min)

Activity - Michaelis-Menten

Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Isopropanol

2 % v/v

3.6

2.6

1000 * 1/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in 1000 * 1/min)

Activity - Michaelis-Menten

Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Acetonitrile

5 % v/v

3.6

2.1

1000 * 1/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in 1000 * 1/min)

Activity - Michaelis-Menten

Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1-Propanol

1 % v/v

3.6

2.9

1000 * 1/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in 1000 * 1/min)

Activity - Michaelis-Menten

Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Dimethylformamide (DMF)

6 % v/v

3.6

1.4

1000 * 1/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in 1000 * 1/min)

Activity - Michaelis-Menten

Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

1,4-Dioxane

6 % v/v

3.6

0.9

1000 * 1/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in 1000 * 1/min)

Activity - Michaelis-Menten

Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Ethanol

5 % v/v

3.6

2.6

1000 * 1/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in 1000 * 1/min)

Activity - Michaelis-Menten

Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

0.5 % v/v

3.6

2.3

1000 * 1/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in 1000 * 1/min)

Activity - Michaelis-Menten

Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Pyridine

0.5 % v/v

3.6

2.1

1000 * 1/min

Direct

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

Phenanthridine

Phenanthridinone

—

Classical aqueous control (in 1000 * 1/min)

Stability - C50

Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

—

1.5

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Tetrahydrofuran (THF)

—

0.5

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Tetrahydrofuran (THF)

—

0.5

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Dimethylformamide (DMF)

—

20.0

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Dimethylformamide (DMF)

—

13.0

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Dimethylformamide (DMF)

—

12.0

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

1-Propanol

—

2.0

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

1-Propanol

—

2.0

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

1-Propanol

—

1.5

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Isopropanol

—

5.0

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Isopropanol

—

5.0

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Isopropanol

—

4.0

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

1,4-Dioxane

—

10.0

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

1,4-Dioxane

—

9.0

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

1,4-Dioxane

—

8.0

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Pyridine

—

0.5

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Pyridine

—

0.5

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Pyridine

—

0.5

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Methanol

—

0.5

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Methanol

—

0.5

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Methanol

—

1.5

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Acetonitrile

—

5.0

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Acetonitrile

—

10.0

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Acetonitrile

—

15.0

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Ethanol

—

17.0

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

25°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Ethanol

—

15.0

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

35°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - C50

Ethanol

—

10.0

% (v/v)

Digital

Continuous

67 mM Sorenson phosphate buffer, 0.1 MM EDTA

7.4

45°C

20 µM Phenanthridine

Phenanthridinone

—

Not applicable control (in % (v/v))

Stability - Incubation

Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

Pyridine

0.5 % v/v

98.0

0.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

Stability - Incubation

Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

1,4-Dioxane

6 % v/v

98.0

21.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

Stability - Incubation

Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

Tetrahydrofuran (THF)

0.5 % v/v

98.0

43.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

Stability - Incubation

Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

Ethanol

5 % v/v

98.0

46.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

Stability - Incubation

Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

1-Propanol

1 % v/v

98.0

56.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

Stability - Incubation

Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

Isopropanol

2 % v/v

98.0

60.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

Stability - Incubation

Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

Dimethylformamide (DMF)

6 % v/v

98.0

63.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

Stability - Incubation

Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

Methanol

0.5 % v/v

98.0

63.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

Stability - Incubation

Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

Acetonitrile

5 % v/v

98.0

63.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

Stability - Incubation

Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

Pyridine

0.5 % v/v

100.0

0.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

Stability - Incubation

Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

Ethanol

5 % v/v

100.0

7.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

Stability - Incubation

Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

Dimethylformamide (DMF)

6 % v/v

100.0

8.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

Stability - Incubation

Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

Isopropanol

2 % v/v

100.0

12.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

Stability - Incubation

Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

Tetrahydrofuran (THF)

0.5 % v/v

100.0

13.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

Stability - Incubation

Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

1-Propanol

1 % v/v

100.0

20.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

Stability - Incubation

Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

Methanol

0.5 % v/v

100.0

52.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

Stability - Incubation

Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

1,4-Dioxane

6 % v/v

100.0

52.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

Stability - Incubation

Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase

Acetonitrile

5 % v/v

100.0

54.0

Digital

Continuous

Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA

Incubation: 7.4, Assay: 4

Incubation: 35°C, Assay: 35°C

20 µM Phenanthridine

Phenanthridinone

—

Water-incubated control (in %), activity measured in aqueous phase

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity — Michaelis-Menten

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — C₅₀

One bar per measurement. Colour = solvent, shade = temperature. Hover for details.

Structure

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Aldehyde oxidase 1 from Oryctolagus cuniculus (rabbit)

Enzyme Description

Sequence

Experimental Data (243 measurements)

Visualization : Activity — Classical

Visualization : Activity — Michaelis-Menten

Visualization : Stability — Incubation

Visualization : Stability — C50

Structure

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Visualization : Stability — C₅₀