Esterase (gene EstLiu) from Zunongwangia profunda

Enzyme Description

Species
Zunongwangia profunda β†—
Extremophile
Yes psychrophilic (and "halo-tolerant" enzyme)
EC Number
3.1.1.1 β†—

Sequence

Length: 293 amino acids
MKTKIIAFLGLFVAVFQLSAQDEVIKLYDGKAPGSEDWNWEEAQKFSDLFKTEVVYNVTDPSLLVFKPETPNGTAIIIAPGGGFQTLSINREGIDLAKELASKGVTAFVLKYRLVHSKTDSPAKELMESLKDRADFDAKRVKIKKLAGQDMQAALKYVRDNAAQFNIAKDKVGVIGFSAGATVALESVLRSASKATLPNFAASLYGGPSDDIMAQDIPKEIPLFIAAASDDQLKLAPKSIALYNKWLAAGYPVELHMYAKGGHGFGMGTQNLPVDTWYKRYEDWLTQYKYLNK
Mohammad Asadur Rahman et al. (2016) β€” Characterization of a novel cold active and salt tolerant esterase from Zunongwangia profunda
Enzyme and Microbial Technology  Β· doi:10.1016/j.enzmictec.2015.12.013 β†—  Β· Stability - Incubation
42 measurements ▼
Database ID
UniProt: D5BL39 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (42 measurements)

42 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 20 % v/v 100 70 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 40 % v/v 100 62 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 50 % v/v 100 39 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethylene Glycol 5 % v/v 100 120 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethylene Glycol 10 % v/v 100 101 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethylene Glycol 20 % v/v 100 97 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethylene Glycol 30 % v/v 100 80 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethylene Glycol 40 % v/v 100 98 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethylene Glycol 50 % v/v 100 79 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 5 % v/v 100 96 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 10 % v/v 100 91 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 30 % v/v 100 89 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 30 % v/v 100 26 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 40 % v/v 100 28 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 50 % v/v 100 14 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Propanol 5 % v/v 100 103 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Propanol 10 % v/v 100 94 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Propanol 20 % v/v 100 64 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Propanol 30 % v/v 100 20 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Propanol 40 % v/v 100 9 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
β€Ή Prev
1 2 3
Next β€Ί

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*