Esterase (gene EstLiu) from Zunongwangia profunda

Enzyme Description

Species
Zunongwangia profunda β†—
Extremophile
Yes psychrophilic (and "halo-tolerant" enzyme)
EC Number
3.1.1.1 β†—

Sequence

Length: 293 amino acids
MKTKIIAFLGLFVAVFQLSAQDEVIKLYDGKAPGSEDWNWEEAQKFSDLFKTEVVYNVTDPSLLVFKPETPNGTAIIIAPGGGFQTLSINREGIDLAKELASKGVTAFVLKYRLVHSKTDSPAKELMESLKDRADFDAKRVKIKKLAGQDMQAALKYVRDNAAQFNIAKDKVGVIGFSAGATVALESVLRSASKATLPNFAASLYGGPSDDIMAQDIPKEIPLFIAAASDDQLKLAPKSIALYNKWLAAGYPVELHMYAKGGHGFGMGTQNLPVDTWYKRYEDWLTQYKYLNK
Mohammad Asadur Rahman et al. (2016) β€” Characterization of a novel cold active and salt tolerant esterase from Zunongwangia profunda
Enzyme and Microbial Technology  Β· doi:10.1016/j.enzmictec.2015.12.013 β†—  Β· Stability - Incubation
42 measurements ▼
Database ID
UniProt: D5BL39 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (42 measurements)

42 measurements β€” page 2 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Propanol 50 % v/v 100 4 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 50 % v/v 100 103 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 10 % v/v 100 114 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 20 % v/v 100 119 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 30 % v/v 100 102 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 40 % v/v 100 68 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 50 % v/v 100 81 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 5 % v/v 100 133 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 10 % v/v 100 120 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 20 % v/v 100 153 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 30 % v/v 100 145 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 40 % v/v 100 138 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 5 % v/v 100 155 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 5 % v/v 100 111 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10 % v/v 100 87 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 20 % v/v 100 82 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 30 % v/v 100 72 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 40 % v/v 100 68 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50 % v/v 100 67 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 5 % v/v 100 117 % Digital Continuous Incubation: ?, Assay: 50 mM Tris-HCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 30Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
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Visualization : Stability β€” Incubation

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