Thermolysin from Bacillus thermoproteolyticus
Enzyme Description
Species
Extremophile
Yes
"thermophilic" organism
EC Number
Sequence
ITGTSTVGVGRGVLGDQKNINTTYSTYYYLQDNTRGNGIFTYDAKYRTTLPGSLWADADNQFFASYDAPAVDAHYYAGVTYDYYKNVHNRLSYDGNNAAIRSSVHYSQGYNNAFWNGSQMVYGDGDGQTFIPLSGGIDVVAHELTHAVTDYTAGLIYQNESGAINEAISDIFGTLVEFYANKNPDWEIGEDVYTPGISGDSLRSMSDPAKYGDPDHYSKRYTGTQDNGGVHINSGIINKAAYLISQGGTHYGVSVVGIGRDKLGKIFYRALTQYLTPTSNFSQLRAAAVQSATDLYGSTSQEVASVKQAFDAVGVK
Kohei Oda et al. (1996)
β A Novel Alcohol Resistant Metalloproteinase, Vimelysin, from Vibrio sp. T1800: Purification and Characterization
Bioscience, Biotechnology, and Biochemistry
Β· doi:10.1271/bbb.60.463 β
Β· Activity - Classical
▼
Database ID
UniProt: P00800 β
Sequence Annotation
Inferred - from protein name (first 232 residues from UniProt sequence absent from the mature protein)
Protein Source
Commercially purchased
Experimental Data (22 measurements)
22 measurements β page 2 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Extraction method | Data type | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent | Methanol | 30 % (v/v) | 100 | 6 | % | Digital | Continuous | 0.1 M MES | 6.5 | ? | 1 mM FAGLA (FA-Gly-DL-Leu-NH2) | Furylacryloyl-Gly , Leu-NH2 | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent | Methanol | 20 % (v/v) | 100 | 12 | % | Digital | Continuous | 0.1 M MES | 6.5 | ? | 1 mM FAGLA (FA-Gly-DL-Leu-NH2) | Furylacryloyl-Gly , Leu-NH2 | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume. β β β Reference value. Hover for details.
Guillermo R. Castro et al. (1999)
β Enzymatic activities of proteases dissolved in organic solvents
Enzyme and Microbial Technology
Β· doi:10.1016/S0141-0229(99)00099-X β
Β· Activity - Michaelis-Menten
▼
Database ID
UniProt: P00800 β
Sequence Annotation
Inferred - from protein name (first 232 residues from UniProt sequence absent from the mature protein)
Protein Source
Commercially purchased
Experimental Data (3 measurements)
3 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Extraction method | Data type | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Michaelis-Menten | Km (mM) measured by HPLC in the presence of organic solvent | Glycerol | 99 % (v/v) | 2.5 | 41.4 | mM | Direct | Continuous | 20 mM phosphate buffer | 7.8 | 37Β°C | Phenyl acetate | Acetic Acid , Phenol | β | β | Classical aqueous control (in mM) |
| Activity - Michaelis-Menten | Vmax (Β΅M/s mg) measured by HPLC in the presence of organic solvent | Glycerol | 99 % (v/v) | 0.5 | 0.48 | Β΅M/(s * mg) | Direct | Continuous | 20 mM phosphate buffer | 7.8 | 37Β°C | Phenyl acetate | Acetic Acid , Phenol | β | β | Classical aqueous control (in microM/(s * mg)) |
| Activity - Michaelis-Menten | Vm/Km (1/s mg) measured by HPLC in the presence of organic solvent | Glycerol | 99 % (v/v) | 0.2 | 0.012 | 1/(s * mg) | Direct | Continuous | 20 mM phosphate buffer | 7.8 | 37Β°C | Phenyl acetate | Acetic Acid , Phenol | β | β | Classical aqueous control (in 1/(s * mg)) |
Visualization : Activity β Michaelis-Menten
One bar per measurement. Colour = solvent, shade = solvent volume. β β β Reference value. Hover for details.
Mohammad Pazhang et al. (2006)
β Effects of water-miscible solvents and polyhydroxy compounds on the structure and enzymatic activity of thermolysin
Journal of Biotechnology
Β· doi:10.1016/j.jbiotec.2006.05.017 β
Β· Activity - Classical Activity - Michaelis-Menten Stability - Half-life
▼
Database ID
UniProt: P00800 β
Sequence Annotation
Inferred - from protein name (first 232 residues from UniProt sequence absent from the mature protein)
Protein Source
Commercially purchased
Experimental Data (68 measurements)
68 measurements β page 1 of 4
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Extraction method | Data type | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | 1-Propanol | 5 % (v/v) | 100.0 | 23.0 | % | Digital | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.005 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | 1-Propanol | 1 % (v/v) | 100.0 | 81.0 | % | Digital | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.005 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20 % (v/v) | 100.0 | 20.0 | % | Digital | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.005 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10 % (v/v) | 100.0 | 49.0 | % | Digital | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.005 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 5 % (v/v) | 100.0 | 69.0 | % | Digital | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.005 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 1 % (v/v) | 100.0 | 89.0 | % | Digital | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.005 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 20 % (v/v) | 100.0 | 17.0 | % | Digital | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.005 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 10 % (v/v) | 100.0 | 43.0 | % | Digital | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.005 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 5 % (v/v) | 100.0 | 65.0 | % | Digital | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.005 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Isopropanol | 20 % (v/v) | 100.0 | 1.0 | % | Digital | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.005 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Isopropanol | 10 % (v/v) | 100.0 | 7.0 | % | Digital | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.005 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Isopropanol | 5 % (v/v) | 100.0 | 22.0 | % | Digital | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.005 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Isopropanol | 1 % (v/v) | 100.0 | 65.0 | % | Digital | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.005 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | 1-Propanol | 20 % (v/v) | 100.0 | 1.0 | % | Digital | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.005 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | 1-Propanol | 10 % (v/v) | 100.0 | 3.0 | % | Digital | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.005 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 1 % (v/v) | 100.0 | 90.0 | % | Digital | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.005 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Michaelis-Menten | V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | 1-Propanol | 133 Millimolar | 1104.0 | 630.0 | amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein | Direct | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | Casein | free amino acids , Peptides | β | β | Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein) |
| Activity - Michaelis-Menten | V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | 1-Propanol | 106.6 Millimolar | 1104.0 | 747.0 | amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein | Direct | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | Casein | free amino acids , Peptides | β | β | Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein) |
| Activity - Michaelis-Menten | V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | 1-Propanol | 65.6 Millimolar | 1104.0 | 870.0 | amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein | Direct | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | Casein | free amino acids , Peptides | β | β | Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein) |
| Activity - Michaelis-Menten | V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | 1-Propanol | 26.6 Millimolar | 1104.0 | 988.0 | amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein | Direct | Continuous | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | Casein | free amino acids , Peptides | β | β | Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume. β β β Reference value. Hover for details.
Visualization : Activity β Michaelis-Menten
One bar per measurement. Colour = solvent, shade = solvent volume. β β β Reference value. Hover for details.
Visualization : Stability β Half-life
One bar per measurement. Colour = solvent, shade = solvent volume. β β β Reference value. Hover for details.
Hiroyasu Ogino et al. (2007)
β Stabilities and Conformational Transitions of Various Proteases in the Presence of an Organic Solvent
Biotechnology Progress
Β· doi:10.1021/bp060252p β
Β· Stability - Half-life
▼
Database ID
UniProt: P00800 β
Sequence Annotation
Inferred - from protein name (first 232 residues from UniProt sequence absent from the mature protein)
Protein Source
Commercially purchased
Experimental Data (1 measurement)
1 measurement
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Extraction method | Data type | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Half-life | Half-life (in hours) at 30Β°C measured using absorbance spectrophotometry (free aromatic residues absorbance measurement, 280 nm) | Methanol | 25 % (v/v) | 416.0 | 98.1 | hours | Direct | Continuous | Incubation: 10 mM sodium phosphate buffer, Assay: 50 mM borax-HCl | Incubation: ?, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | β | Classical aqueous control (in hours) |
Visualization : Stability β Half-life
One bar per measurement. Colour = solvent, shade = solvent volume. β β β Reference value. Hover for details.
Bassem Jaouadi et al. (2013)
β Biochemical and molecular characterization of Pseudomonas aeruginosa CTM50182 organic solvent-stable elastase
International Journal of Biological Macromolecules
Β· doi:10.1016/j.ijbiomac.2013.05.019 β
Β· Stability - Incubation
▼
Database ID
UniProt: P00800 β
Sequence Annotation
Inferred - from protein name (232 first residues from UniProt sequence absent from the mature protein)
Protein Source
Commercially purchased
Experimental Data (40 measurements)
40 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Extraction method | Data type | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | 1-Hexanol | 60 % (v/v) | 100 | 52 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | n-Decane | 60 % (v/v) | 100 | 77 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Isooctane | 60 % (v/v) | 100 | 108 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | n-Octane | 60 % (v/v) | 100 | 112 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | n-Heptane | 60 % (v/v) | 100 | 127 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | n-Hexane | 60 % (v/v) | 100 | 135 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Cyclohexane | 60 % (v/v) | 100 | 125 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Toluene | 60 % (v/v) | 100 | 72 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Benzene | 60 % (v/v) | 100 | 60 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Chloroform | 60 % (v/v) | 100 | 83 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | n-Hexadecane | 60 % (v/v) | 100 | 95 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | 1-Butanol | 60 % (v/v) | 100 | 38 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Ethyl Acetate | 60 % (v/v) | 100 | 0 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Isopropanol | 60 % (v/v) | 100 | 91 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Isopropanol | 60 % (v/v) | 100 | 88 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Acetonitrile | 60 % (v/v) | 100 | 0 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Ethanol | 60 % (v/v) | 100 | 71 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Methanol | 60 % (v/v) | 100 | 75 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Dimethylformamide (DMF) | 60 % (v/v) | 100 | 115 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 60 % (v/v) | 100 | 127 | % | Direct | Continuous | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume. β β β Reference value. Hover for details.
Akli Ouelhadj et al. (2020)
β Identification and homology modeling of a new biotechnologically compatible serine alkaline protease from moderately halotolerant Gracilibacillus boraciitolerans strain LO15
International Journal of Biological Macromolecules
Β· doi:10.1016/j.ijbiomac.2020.07.266 β
Β· Activity - Classical
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Database ID
UniProt: P00800 β
Sequence Annotation
Inferred - from protein name (first 232 residues from UniProt sequence absent from the mature protein)
Protein Source
Commercially purchased
Experimental Data (10 measurements)
10 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Extraction method | Data type | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent | n-Decane | 25 % (v/v) | 100 | 90 | % | Digital | Continuous | 83 mM glycine-NaOH buffer | 10 | 60Β°C | 0.008 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent | n-Octane | 25 % (v/v) | 100 | 132 | % | Digital | Continuous | 83 mM glycine-NaOH buffer | 10 | 60Β°C | 0.008 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent | n-Hexane | 25 % (v/v) | 100 | 101 | % | Digital | Continuous | 83 mM glycine-NaOH buffer | 10 | 60Β°C | 0.008 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent | Toluene | 25 % (v/v) | 100 | 92 | % | Digital | Continuous | 83 mM glycine-NaOH buffer | 10 | 60Β°C | 0.008 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent | Chloroform | 25 % (v/v) | 100 | 66 | % | Digital | Continuous | 83 mM glycine-NaOH buffer | 10 | 60Β°C | 0.008 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent | 1-Butanol | 25 % (v/v) | 100 | 15 | % | Digital | Continuous | 83 mM glycine-NaOH buffer | 10 | 60Β°C | 0.008 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent | Isopropanol | 25 % (v/v) | 100 | 91 | % | Digital | Continuous | 83 mM glycine-NaOH buffer | 10 | 60Β°C | 0.008 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent | Acetonitrile | 25 % (v/v) | 100 | 0 | % | Digital | Continuous | 83 mM glycine-NaOH buffer | 10 | 60Β°C | 0.008 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent | Methanol | 25 % (v/v) | 100 | 75 | % | Digital | Continuous | 83 mM glycine-NaOH buffer | 10 | 60Β°C | 0.008 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 25 % (v/v) | 100 | 127 | % | Digital | Continuous | 83 mM glycine-NaOH buffer | 10 | 60Β°C | 0.008 Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume. β β β Reference value. Hover for details.