Esterase (gene EstSP) from a Goa solar saltern metagenomic library

Enzyme Description

Species
Na (Goa solar saltern metagenomic library)
Extremophile
Yes probably halophilic organism (solar saltern), halotolerant enzyme
EC Number
3.1.1.1 β†—

Sequence

Length: 337 amino acids
MLGGLEQPMLHQIADVDSREEALAIANSESALAEEAALIELMQAMDDESVHSSAGLVISEHHINSEPDNNSINVRVIRPDTDAVLPCILYLHGGAMMSMSYDLGTYRAWGKVLAQQGICVAMVDFRNSLRPSTTEEVAPFPAGLNDCVSGYHWVRTNASALKIDPESVLIAGDSGGGNLAIATTMRLVDAGQPPLGLYALCPYILGQWPNADYPSSEEFEGYLISVHNNHATMAYGIEAFERRDRYAWPGFATAENLQGFPPSMISVNECDPLRDEGIAFYRKLLEAGVQAHCRQLMGTVHANEMFTTVFPELTRMTARDMVGWVRECQLLTGSAPA
G. Jayanath et al. (2018) β€” A novel solvent tolerant esterase of GDSGG motif subfamily from solar saltern through metagenomic approach: Recombinant expression and characterization
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2018.06.057 β†—  Β· Stability - Incubation
64 measurements ▼
Database ID
UniParc: UPI000B54DD3F β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli Rosetta-gami B(DE3) pLysS

Experimental Data (64 measurements)

64 measurements β€” page 2 of 4
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10 % v/v 100.0 93.66 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 20 % v/v 100.0 44.89 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Butanol 10 % v/v 100.0 129.83 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Butanol 20 % v/v 100.0 121.96 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Xylene 10 % v/v 100.0 84.26 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Xylene 20 % v/v 100.0 41.6 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 10 % v/v 100.0 0.0 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 20 % v/v 100.0 0.0 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethylformamide (DMF) 10 % v/v 100.0 20.86 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethylformamide (DMF) 20 % v/v 100.0 8.43 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase n-Hexane 10 % v/v 100.0 83.29 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase n-Hexane 20 % v/v 100.0 45.97 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 10 % v/v 100.0 125.51 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 20 % v/v 100.0 126.93 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 10 % v/v 100.0 145.09 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 20 % v/v 100.0 126.54 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10 % v/v 100.0 114.07 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 20 % v/v 100.0 102.2 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Butanol 10 % v/v 100.0 145.98 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Butanol 20 % v/v 100.0 135.73 % Direct Continuous Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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