N-terminal truncated (20 residues) esterase (gene estA1) from Alteromonas sp. 39-G1

Enzyme Description

Species
Alteromonas sp. 39-G1 β†—
Extremophile
No
EC Number
3.1.1.1 β†—

Sequence

Length: 359 amino acids
ASTSGASTHNTDTASASKQKADTVKISNESPLTVPAKTLPLPSASSDELKSAISQYPMPSVDEVINNTPQSIEQWRELIQIRNADQKKKIKKMRKQFDVDVSLEKINGVPVRRLTPKTIAPEFKNKVFIDVHGGAYVFFSGLPSIEESLLIAHRVGITVISIDYSMPPHAPFPAALNDVVSVYSSVAAEHGAQNLFIGGTSAGAGLVLAAVQTLIADKQPLPAAVYAGTPWADLTKTGDTLYTNEGVDRILVTYQGFLEAAANLYAGSESLTHSSISPLYGNFDGFPPTFLISGTRDMFLSDTVRVNRKLRDAQVRTQLEVFEGLSHADYVVAYETPESHSVYQELKQFLLSVCTKSSD
Seok-Jae Won et al. (2020) β€” Characterization of Novel Salt-Tolerant Esterase Isolated from the Marine Bacterium Alteromonas sp. 39-G1
Journal of Microbiology and Biotechnology  Β· doi:10.4014/jmb.1907.07057 β†—  Β· Stability - Incubation
24 measurements ▼
Database ID
UniParc: UPI000C1070DA β†—
Sequence Annotation
Explicit (20 first residues deleted)
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (24 measurements)

24 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 30 % v/v 100 66 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase n-Hexane 50 % v/v 100 159 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Cyclohexane 50 % v/v 100 135 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 50 % v/v 100 0 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 50 % v/v 100 0 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 50 % v/v 100 0 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 50 % v/v 100 0 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 50 % v/v 100 0 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50 % v/v 100 98 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase n-Hexane 30 % v/v 100 121 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Cyclohexane 30 % v/v 100 131 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 30 % v/v 100 0 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 15 % v/v 100 106 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 30 % v/v 100 7 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 30 % v/v 100 0 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 30 % v/v 100 94 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 30 % v/v 100 101 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase n-Hexane 15 % v/v 100 103 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Cyclohexane 15 % v/v 100 101 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 15 % v/v 100 102 % Direct Continuous Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 40Β°C 0.1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
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Visualization : Stability β€” Incubation

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Structure

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