Ξ²-xylosidase Dt-2286 from Dictyoglomus turgidum
Enzyme Description
Extremophile
Yes
"thermophilic" organism
EC Number
Sequence
MEEKEILKKVRDLISKMTLEEKIAQLQSVFGKELVDESGNFSEEKAEKLLKNGIGQISRVAGEKGMDPERAVELANKIQKFLKEKTRLGIPAIIHEECLSGFMAKGATVFPQAIGMASTFEPELIRRVSDVIRQHMRAANVHQGLSPVLDIPRDPRWGRTEETFGEDPYLVSRMAAEYVKGLQGEDWREGIIATVKHFTAYGISEGARNLGPAKVGERELREVFLFPFEVAIKEGQAGSLMNAYHEIDGVPCASSKFLLTKILRWEWGFKGYVVSDYIAIRMLENFHRVAKDAKEAAVLALEAGIDIELPSVDCYGEPLIQAVKEGLISEEVINASVERVLRAKFMLGLFDGDLEKDPKKVYDIFDKPEFRELSREVARRSIVLLKNDGILPLSKNIRTVAVIGPNADNPRNLHGDYSYTAHIPSVSETLEGVKIPEECAVRTVSILEGIKNKVSAETQVLYAKGCEILSDSKEGFDEAIEIAKRADVIIAVMGEESGLFHRGISGEGNDRTTLELFGIQRDLLRELHKLGKPIVLVLVNGRPQALKWEHENLNAILEAWYPGEEGGDAVADVIFGDYNPSGKLPISFPAVTGQVPVYYNRKPSAFTDYVEESAKPLYPFGHGLSYTTFEYSNLKIHPEKVNALEKVEISFTIKNTGVREGEEVVQLYVHDQVASLERPVKELKGFKKIHLKPGESKRVTFILYPEQLAFYDEFMRFVVEKGIFEIMIGSSSEDIRLTGTFEVLETKVITEKRKFASEIKVE
Xinyi Tong et al. (2021)
β High-level expression of a novel multifunctional GH3 family Ξ²-xylosidase/Ξ±-arabinosidase/Ξ²-glucosidase from Dictyoglomus turgidum in Escherichia coli
Bioorganic Chemistry
Β· doi:10.1016/j.bioorg.2021.104906 β
Β· Activity - Classical
▼
Database ID
UniProt: B8E050 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (24 measurements)
24 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Extraction method | Data type | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 25 % v/v | 100 | 102 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 50 % v/v | 100 | 12 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 40 % v/v | 100 | 46 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 30 % v/v | 100 | 86 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 25 % v/v | 100 | 95 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20 % v/v | 100 | 95 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 15 % v/v | 100 | 94 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10 % v/v | 100 | 95 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 5 % v/v | 100 | 94 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 50 % v/v | 100 | 19 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 40 % v/v | 100 | 51 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 30 % v/v | 100 | 98 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 5 % v/v | 100 | 102 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 20 % v/v | 100 | 103 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 15 % v/v | 100 | 102 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 10 % v/v | 100 | 102 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 5 % v/v | 100 | 101 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 50 % v/v | 100 | 11 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 40 % v/v | 100 | 81 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 30 % v/v | 100 | 100 | % | Digital | Continuous | 50 mM sodium phosphate buffer | 5 | 90Β°C | 1 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.