β-xylosidase Dt-2286 from Dictyoglomus turgidum

Enzyme Description

Species

Dictyoglomus turgidum DSM6724 ↗

Extremophile

Yes "thermophilic" organism

EC Number

3.2.1.37 ↗

Sequence

Length: 762 amino acids

MEEKEILKKVRDLISKMTLEEKIAQLQSVFGKELVDESGNFSEEKAEKLLKNGIGQISRVAGEKGMDPERAVELANKIQKFLKEKTRLGIPAIIHEECLSGFMAKGATVFPQAIGMASTFEPELIRRVSDVIRQHMRAANVHQGLSPVLDIPRDPRWGRTEETFGEDPYLVSRMAAEYVKGLQGEDWREGIIATVKHFTAYGISEGARNLGPAKVGERELREVFLFPFEVAIKEGQAGSLMNAYHEIDGVPCASSKFLLTKILRWEWGFKGYVVSDYIAIRMLENFHRVAKDAKEAAVLALEAGIDIELPSVDCYGEPLIQAVKEGLISEEVINASVERVLRAKFMLGLFDGDLEKDPKKVYDIFDKPEFRELSREVARRSIVLLKNDGILPLSKNIRTVAVIGPNADNPRNLHGDYSYTAHIPSVSETLEGVKIPEECAVRTVSILEGIKNKVSAETQVLYAKGCEILSDSKEGFDEAIEIAKRADVIIAVMGEESGLFHRGISGEGNDRTTLELFGIQRDLLRELHKLGKPIVLVLVNGRPQALKWEHENLNAILEAWYPGEEGGDAVADVIFGDYNPSGKLPISFPAVTGQVPVYYNRKPSAFTDYVEESAKPLYPFGHGLSYTTFEYSNLKIHPEKVNALEKVEISFTIKNTGVREGEEVVQLYVHDQVASLERPVKELKGFKKIHLKPGESKRVTFILYPEQLAFYDEFMRFVVEKGIFEIMIGSSSEDIRLTGTFEVLETKVITEKRKFASEIKVE

Xinyi Tong et al. (2021) — High-level expression of a novel multifunctional GH3 family β-xylosidase/α-arabinosidase/β-glucosidase from Dictyoglomus turgidum in Escherichia coli

Bioorganic Chemistry · doi:10.1016/j.bioorg.2021.104906 ↗ · Activity - Classical

24 measurements ▼

Database ID

UniProt: B8E050 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (24 measurements)

24 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	25 % v/v	100	91	%	Digital	Continuous	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20 % v/v	100	104	%	Digital	Continuous	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	15 % v/v	100	104	%	Digital	Continuous	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	10 % v/v	100	106	%	Digital	Continuous	50 mM sodium phosphate buffer	5	90°C	1 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Ethanol

25 % v/v

100

102

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50 % v/v

100

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40 % v/v

100

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30 % v/v

100

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25 % v/v

100

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15 % v/v

100

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

5 % v/v

100

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Ethanol

50 % v/v

100

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Ethanol

40 % v/v

100

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Ethanol

30 % v/v

100

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Methanol

5 % v/v

100

102

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Ethanol

20 % v/v

100

103

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Ethanol

15 % v/v

100

102

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Ethanol

10 % v/v

100

102

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Ethanol

5 % v/v

100

101

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Methanol

50 % v/v

100

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Methanol

40 % v/v

100

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Methanol

30 % v/v

100

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Methanol

25 % v/v

100

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Methanol

20 % v/v

100

104

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Methanol

15 % v/v

100

104

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent

Methanol

10 % v/v

100

106

Digital

Continuous

50 mM sodium phosphate buffer

90°C

1 mM p-nitrophenyl β-D-xylopyranoside

D-Xylose, p-Nitrophenol

—

Classical aqueous control (in %)

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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β-xylosidase Dt-2286 from Dictyoglomus turgidum

Enzyme Description

Sequence

Experimental Data (24 measurements)

Visualization : Activity — Classical

Structure

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