Chitosinase (gene ChoK) from Bacillus sp. CK4

Enzyme Description

Species
Bacillus sp. CK4 β†—
Extremophile
Yes "thermophilic" organism
EC Number
3.2.1.132 β†—

Sequence

Length: 273 amino acids
MREADWWGKAAISLLVFTMFFTLMMSETVFAAGLNKDQKRRAEQLRRICEDGTTEMRYPYVARLDDARPSTCGPAGVTTATRDGFEVVPVYKQAVANKKLPNYLAGLRRLEKEASDDTSKLKGPASAWKSLADDKAFRAAQDGVNDQVYYQPAMERSDNAGLTTALARAVMYHTVRQRGDGDDGDSRYALIKRTPKGAGGSPKEGIDEQKCLNKFSHVRYDDLMNGANHDRRDEWRESVGRVHVLRSIANQNNYNLNGGIHVRSHEYGNGVIK
Ho-Geun YOON et al. (2001) β€” Thermostable Chitosanase from Bacillus sp. Strain CK4: Its Purification, Characterization, and Reaction Patterns
Bioscience, Biotechnology, and Biochemistry  Β· doi:10.1271/bbb.65.802 β†—  Β· Stability - Incubation
30 measurements ▼
Database ID
UniProt: Q9RC18 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, bacterium Bacillus sp. CK4

Experimental Data (30 measurements)

30 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Ethyl Acetate 10 % v/v 100 90 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetonitrile 50 % v/v 100 20 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetonitrile 40 % v/v 100 47 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetonitrile 30 % v/v 100 71 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetonitrile 20 % v/v 100 89 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetonitrile 10 % v/v 100 99 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Chloroform 50 % v/v 100 30 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Chloroform 40 % v/v 100 50 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Chloroform 30 % v/v 100 66 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Chloroform 20 % v/v 100 82 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Chloroform 10 % v/v 100 99 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Ethyl Acetate 50 % v/v 100 10 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Ethyl Acetate 40 % v/v 100 33 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Ethyl Acetate 30 % v/v 100 55 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Ethyl Acetate 20 % v/v 100 76 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Ethanol 10 % v/v 100 100 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetone 50 % v/v 100 43 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetone 40 % v/v 100 65 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetone 30 % v/v 100 83 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 55Β°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase Acetone 20 % v/v 100 92 % Digital Continuous Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 60Β°C 0.25% soluble Chitosan Reducing sugars β€” Incubation: 400 rpm Non-incubated control (in %), assay in aqueous phase
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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