Amylopullulanase (gene Amy1136) from Cohnella sp. A01

Enzyme Description

Species
Cohnella sp. A01 β†—
Extremophile
Yes "thermophilic" organism
EC Number
3.2.1.41 β†—, 3.2.1.1 β†—

Sequence

Length: 721 amino acids
MSLKKKVTAGTLAASLAMTALAGIPLSSKGLAQKLGLNNIASAASISGSQLGFFASQLKSALSASGAVYTKSVADSVYAYFDSVSSVTYVTYDGVTTPTSDIIKPVKDKIKFKEDSGLQLTSEDEKNLELVFATLAKFTFELEAGKLDALQSNESVRATLRKIAQGAGVDGLTVEDLNGLLFGAKGIEAIAWQELANSSQSLPDFLNNDEARNDFVKRVFKAYLNTNEDLAKALKAYEVNEEDLARVVSNFRDAVGASNFNKAAAALVEAYINVKSSPVSGGGSGVSNNVLDNARKELAAIKAKLASASDAEKQALIDQALKIAAEAIAKLAQFDASSLVKEVDGKAVVSLSLANLSSLFSSVEQLKAALADAIGADNAAKLAKPTLVIDLGKTTLKSSVVNLAKDISAKAKELNFGWIAIKVSGATTKFPLSTLGGDVIYTIDRTAASASQTGGRPAVTDVFNYSLSVNGTVYDKFNEPVEIQLPVTNTSGVDTELLSVAKLDNGSLSIEGGVYGDGVLTEQRYTFSSYVGVENDTFRDVASVQAWAGRQIKVVAAKGAIEGRGDGRFDPRANVTRAEFAKILVSALDLDNPNVTSTKFTDVNPNAWYAPYVAVAAEKGIINGRSATKFDPNAPISRAEIATLIARALQVTKGFSEVKDIDAALANFSDAGKIPSSLKAGVAFAASKNIVIGTNGKFNPNDNASRAEAAVIVYRALNAKA
Faezeh Hasani et al. (2024) β€” Expression optimization and characterization of a novel amylopullulanase from the thermophilic Cohnella sp. A01
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2024.135135 β†—  Β· Activity - Classical
24 measurements ▼
Database ID
β€”
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (24 measurements)

24 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Ethanol 1 % v/v 100 51 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Methanol 2 % v/v 100 49 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Pullulan Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Methanol 1 % v/v 100 82 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Pullulan Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Methanol 2 % v/v 100 55 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Methanol 1 % v/v 100 54 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Acetone 2 % v/v 100 58 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Pullulan Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Acetone 1 % v/v 100 79 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Pullulan Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Acetone 2 % v/v 100 21 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Acetone 1 % v/v 100 55 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Ethanol 2 % v/v 100 76 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Pullulan Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Ethanol 1 % v/v 100 69 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Pullulan Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Ethanol 2 % v/v 100 91 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Glycerol 1 % v/v 100 70 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Isobutanol 2 % v/v 100 94 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Pullulan Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Isobutanol 1 % v/v 100 76 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Pullulan Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Isobutanol 2 % v/v 100 54 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Isobutanol 1 % v/v 100 41 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Starch Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Isopropanol 2 % v/v 100 62 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Pullulan Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Isopropanol 1 % v/v 100 49 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Pullulan Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent Isopropanol 2 % v/v 100 62 % Digital Continuous 50 mM potassium-phosphate buffer 8 50Β°C 0.01 Starch Reducing sugars β€” β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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