Amylopullulanase (gene Amy1136) from Cohnella sp. A01

Enzyme Description

Species

Cohnella sp. A01 ↗

Extremophile

Yes "thermophilic" organism

EC Number

3.2.1.41 ↗, 3.2.1.1 ↗

Sequence

Length: 721 amino acids

MSLKKKVTAGTLAASLAMTALAGIPLSSKGLAQKLGLNNIASAASISGSQLGFFASQLKSALSASGAVYTKSVADSVYAYFDSVSSVTYVTYDGVTTPTSDIIKPVKDKIKFKEDSGLQLTSEDEKNLELVFATLAKFTFELEAGKLDALQSNESVRATLRKIAQGAGVDGLTVEDLNGLLFGAKGIEAIAWQELANSSQSLPDFLNNDEARNDFVKRVFKAYLNTNEDLAKALKAYEVNEEDLARVVSNFRDAVGASNFNKAAAALVEAYINVKSSPVSGGGSGVSNNVLDNARKELAAIKAKLASASDAEKQALIDQALKIAAEAIAKLAQFDASSLVKEVDGKAVVSLSLANLSSLFSSVEQLKAALADAIGADNAAKLAKPTLVIDLGKTTLKSSVVNLAKDISAKAKELNFGWIAIKVSGATTKFPLSTLGGDVIYTIDRTAASASQTGGRPAVTDVFNYSLSVNGTVYDKFNEPVEIQLPVTNTSGVDTELLSVAKLDNGSLSIEGGVYGDGVLTEQRYTFSSYVGVENDTFRDVASVQAWAGRQIKVVAAKGAIEGRGDGRFDPRANVTRAEFAKILVSALDLDNPNVTSTKFTDVNPNAWYAPYVAVAAEKGIINGRSATKFDPNAPISRAEIATLIARALQVTKGFSEVKDIDAALANFSDAGKIPSSLKAGVAFAASKNIVIGTNGKFNPNDNASRAEAAVIVYRALNAKA

Faezeh Hasani et al. (2024) — Expression optimization and characterization of a novel amylopullulanase from the thermophilic Cohnella sp. A01

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.135135 ↗ · Activity - Classical

24 measurements ▼

Database ID

—

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (24 measurements)

24 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Isopropanol	1 % v/v	100	66	%	Digital	Continuous	50 mM potassium-phosphate buffer	8	50°C	0.01 Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Glycerol	2 % v/v	100	57	%	Digital	Continuous	50 mM potassium-phosphate buffer	8	50°C	0.01 Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Glycerol	1 % v/v	100	88	%	Digital	Continuous	50 mM potassium-phosphate buffer	8	50°C	0.01 Pullulan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent	Glycerol	2 % v/v	100	48	%	Digital	Continuous	50 mM potassium-phosphate buffer	8	50°C	0.01 Starch	Reducing sugars	—	—	Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Ethanol

1 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Starch

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Methanol

2 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Pullulan

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Methanol

1 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Pullulan

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Methanol

2 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Starch

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Methanol

1 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Starch

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Acetone

2 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Pullulan

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Acetone

1 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Pullulan

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Acetone

2 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Starch

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Acetone

1 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Starch

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Ethanol

2 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Pullulan

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Ethanol

1 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Pullulan

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Ethanol

2 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Starch

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Glycerol

1 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Starch

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Isobutanol

2 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Pullulan

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Isobutanol

1 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Pullulan

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Isobutanol

2 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Starch

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Isobutanol

1 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Starch

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Isopropanol

2 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Pullulan

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Isopropanol

1 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Pullulan

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Isopropanol

2 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Starch

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Isopropanol

1 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Starch

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Glycerol

2 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Pullulan

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Glycerol

1 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Pullulan

Reducing sugars

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (DNS assay, 530 nm) in the presence of organic solvent

Glycerol

2 % v/v

100

Digital

Continuous

50 mM potassium-phosphate buffer

50°C

0.01 Starch

Reducing sugars

—

Classical aqueous control (in %)

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Amylopullulanase (gene Amy1136) from Cohnella sp. A01

Enzyme Description

Sequence

Experimental Data (24 measurements)

Visualization : Activity — Classical

Structure

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