Lactoperoxidase from Bubalus bubalis (domestic water buffalo)

Enzyme Description

Species
Bubalus bubalis (domestic water buffalo) β†—
Extremophile
No
EC Number
1.11.1.7 β†—

Sequence

Length: 712 amino acids
MWVCLQLPVFLASVTLFEVAASDTIAQAASTTTISDAVSKVKIQVNKAFLDSRTRLKTTLSSEAPTTQQLSEYFKHAKGQTRTAIRNGQVWEESFKRLRRDTTLTNVTDPSLDLTALSWEVGCGAPVPLVKCDENSPYRTITGDCNNRRSPALGAANRALARWLPAEYEDGLALPFGWTQRKTRNGFRVPLAREVSNKIVGYLDEEGVLDQNRSLLFMQWGQIVDHDLDFAPETELGSNEHSKTQCEEYCIQGDNCFPIMFPKNDPKLKTQGKCMPFFRAGFVCPTPPYQSLAREQINAVTSFLDASLVYGSEPSLASRLRNLSSPLGLMAVNQEAWDHGLAYLPFNNKKPSPCEFINTTARVPCFLAGDFRASEQILLATAHTLLLREHNRLARELKKLNPHWNGEKLYQEARKILGAFIQIITFRDYLPIVLGSEMQKWIPPYQGYNNSVDPRISNVFTFAFRFGHMEVPSTVSRLDENYQPWGPEAELPLHTLFFNTWRIIKDGGIDPLTRGLLAKKSKLMNQDKMVTSELRNKLFQPTHKIHGFDLAAINLQRCRDHGMPGYNSWRGFCGLSQPKTLKGLQTVLKNKILAKKLMDLYKTPDNIDIWIGGNAEPMVERGRVGPLLACLLGRQFQQIRDGDRFWWENPGVFTEKQRDSLQKFSFSRLICDNTHITKVPLHAFQANNYPHDFVDCSTVDKLDLSPWASREN
H. Γ–zdemir et al. (2003) β€” Effects of the Water-Miscible Organic Solvents on Lactoperoxidase Purified from Creek-Water Buffalo Milk
Biochemistry (Moscow)  Β· doi:10.1023/a:1023062402300 β†—  Β· Activity - Classical
39 measurements ▼
Database ID
UniProt: A5JUY8 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, eukaryote Bubalus bubalis (domestic water buffalo)

Experimental Data (39 measurements)

39 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Ethanol 0.6 Molar 100 62 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Ethylene Glycol 1.8 Molar 100 82 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Ethylene Glycol 3.5 Molar 100 54 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Ethylene Glycol 4.7 Molar 100 37 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Ethylene Glycol 6.5 Molar 100 15 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Methanol 0.8 Molar 100 87 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Methanol 1.6 Molar 100 73 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Methanol 2.5 Molar 100 60 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Methanol 5 Molar 100 15 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Methanol 6.5 Molar 100 5 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Ethylene Glycol 1.2 Molar 100 90 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Ethanol 1.15 Molar 100 42 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Ethanol 1.75 Molar 100 29 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Ethanol 3.5 Molar 100 6 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Ethanol 4.6 Molar 100 1 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Tetrahydrofuran (THF) 0.2 Molar 100 69 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Tetrahydrofuran (THF) 0.4 Molar 100 49 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Tetrahydrofuran (THF) 0.65 Molar 100 42 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Tetrahydrofuran (THF) 1.25 Molar 100 20 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Tetrahydrofuran (THF) 2.5 Molar 100 3 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

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Structure

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