Lactoperoxidase from Bubalus bubalis (domestic water buffalo)

Enzyme Description

Species
Bubalus bubalis (domestic water buffalo) β†—
Extremophile
No
EC Number
1.11.1.7 β†—

Sequence

Length: 712 amino acids
MWVCLQLPVFLASVTLFEVAASDTIAQAASTTTISDAVSKVKIQVNKAFLDSRTRLKTTLSSEAPTTQQLSEYFKHAKGQTRTAIRNGQVWEESFKRLRRDTTLTNVTDPSLDLTALSWEVGCGAPVPLVKCDENSPYRTITGDCNNRRSPALGAANRALARWLPAEYEDGLALPFGWTQRKTRNGFRVPLAREVSNKIVGYLDEEGVLDQNRSLLFMQWGQIVDHDLDFAPETELGSNEHSKTQCEEYCIQGDNCFPIMFPKNDPKLKTQGKCMPFFRAGFVCPTPPYQSLAREQINAVTSFLDASLVYGSEPSLASRLRNLSSPLGLMAVNQEAWDHGLAYLPFNNKKPSPCEFINTTARVPCFLAGDFRASEQILLATAHTLLLREHNRLARELKKLNPHWNGEKLYQEARKILGAFIQIITFRDYLPIVLGSEMQKWIPPYQGYNNSVDPRISNVFTFAFRFGHMEVPSTVSRLDENYQPWGPEAELPLHTLFFNTWRIIKDGGIDPLTRGLLAKKSKLMNQDKMVTSELRNKLFQPTHKIHGFDLAAINLQRCRDHGMPGYNSWRGFCGLSQPKTLKGLQTVLKNKILAKKLMDLYKTPDNIDIWIGGNAEPMVERGRVGPLLACLLGRQFQQIRDGDRFWWENPGVFTEKQRDSLQKFSFSRLICDNTHITKVPLHAFQANNYPHDFVDCSTVDKLDLSPWASREN
H. Γ–zdemir et al. (2003) β€” Effects of the Water-Miscible Organic Solvents on Lactoperoxidase Purified from Creek-Water Buffalo Milk
Biochemistry (Moscow)  Β· doi:10.1023/a:1023062402300 β†—  Β· Activity - Classical
39 measurements ▼
Database ID
UniProt: A5JUY8 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, eukaryote Bubalus bubalis (domestic water buffalo)

Experimental Data (39 measurements)

39 measurements β€” page 2 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 0.5 Molar 100 57 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Acetonitrile 1.3 Molar 100 50 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Acetonitrile 1.9 Molar 100 34 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Acetonitrile 3.8 Molar 100 8 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Isopropanol 0.4 Molar 100 72 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Isopropanol 0.8 Molar 100 50 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Isopropanol 1.25 Molar 100 41 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Isopropanol 2.5 Molar 100 16 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Isopropanol 3.3 Molar 100 4 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 0.25 Molar 100 76 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Acetonitrile 0.65 Molar 100 74 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 0.7 Molar 100 40 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 1.4 Molar 100 13 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 2.8 Molar 100 1 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Dimethylformamide (DMF) 0.2 Molar 100 70 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Dimethylformamide (DMF) 0.4 Molar 100 46 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Dimethylformamide (DMF) 0.65 Molar 100 28 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Dimethylformamide (DMF) 1.3 Molar 100 2 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 412 nm) in the presence of organic solvent Ethylene Glycol 0.6 Molar 100 95 % Digital Continuous 100 mM Phosphate buffer 6.8 25Β°C 1 mM ABTS, 0.1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) ABTS radical cation , H2O β€” β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

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Structure

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