Lipase from Rhizomucor miehei

Enzyme Description

Species
Rhizomucor miehei β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 363 amino acids
MVLKQRANYLGFLIVFFTAFLVEAVPIKRQSNSTVDSLPPLIPSRTSAPSSSPSTTDPEAPAMSRNGPLPSDVETKYGMALNATSYPDSVVQAMSIDGGIRAATSQEINELTYYTTLSANSYCRTVIPGATWDCIHCDATEDLKIIKTWSTLIYDTNAMVARGDSEKTIYIVFRGSSSIRNWIADLTFVPVSYPPVSGTKVHKGFLDSYGEVQNELVATVLDQFKQYPSYKVAVTGHSLGGATALLCALDLYQREEGLSSSNLFLYTQGQPRVGDPAFANYVVSTGIPYRRTVNERDIVPHLPPAAFGFLHAGEEYWITDNSPETVQVCTSDLETSDCSNSIVPFTSVLDHLSYFGINTGLCT
Wakako Tsuzuki et al. (2003) β€” Polar Organic Solvent Added to an Aqueous Solution Changes Hydrolytic Property of Lipase
Bioscience, Biotechnology, and Biochemistry  Β· doi:10.1271/bbb.67.1660 β†—  Β· Activity - Classical Stability - Incubation
190 measurements ▼
Database ID
UniProt: P19515 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased

Experimental Data (190 measurements)

190 measurements β€” page 7 of 10
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,3-Dioxane 5 % v/v 100.0 50.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,3-Dioxane 10 % v/v 100.0 25.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,3-Dioxane 15 % v/v 100.0 14.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,3-Dioxane 20 % v/v 100.0 6.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,3-Dioxane 25 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,3-Dioxane 30 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,3-Dioxane 35 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,3-Dioxane 40 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,3-Dioxane 45 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,3-Dioxane 50 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,3-Dioxane 60 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,3-Dioxane 70 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,3-Dioxane 80 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,3-Dioxane 90 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent Methanol 10 % v/v 100.0 67.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent Methanol 20 % v/v 100.0 46.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent Methanol 30 % v/v 100.0 36.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent Methanol 40 % v/v 100.0 7.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent Methanol 50 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent Methanol 60 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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