Lipase from Rhizomucor miehei

Enzyme Description

Species

Extremophile

EC Number

3.1.1.3 ↗

Sequence

Length: 363 amino acids

MVLKQRANYLGFLIVFFTAFLVEAVPIKRQSNSTVDSLPPLIPSRTSAPSSSPSTTDPEAPAMSRNGPLPSDVETKYGMALNATSYPDSVVQAMSIDGGIRAATSQEINELTYYTTLSANSYCRTVIPGATWDCIHCDATEDLKIIKTWSTLIYDTNAMVARGDSEKTIYIVFRGSSSIRNWIADLTFVPVSYPPVSGTKVHKGFLDSYGEVQNELVATVLDQFKQYPSYKVAVTGHSLGGATALLCALDLYQREEGLSSSNLFLYTQGQPRVGDPAFANYVVSTGIPYRRTVNERDIVPHLPPAAFGFLHAGEEYWITDNSPETVQVCTSDLETSDCSNSIVPFTSVLDHLSYFGINTGLCT

Wakako Tsuzuki et al. (2003) — Polar Organic Solvent Added to an Aqueous Solution Changes Hydrolytic Property of Lipase

Bioscience, Biotechnology, and Biochemistry · doi:10.1271/bbb.67.1660 ↗ · Activity - Classical Stability - Incubation

190 measurements ▼

Database ID

UniProt: P19515 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Commercially purchased

Experimental Data (190 measurements)

190 measurements — page 1 of 10

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	5 % v/v	100.0	103.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10 % v/v	100.0	109.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	15 % v/v	100.0	150.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20 % v/v	100.0	186.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	25 % v/v	100.0	175.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30 % v/v	100.0	96.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	35 % v/v	100.0	56.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	40 % v/v	100.0	44.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	45 % v/v	100.0	39.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	50 % v/v	100.0	34.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	60 % v/v	100.0	28.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	70 % v/v	100.0	13.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	80 % v/v	100.0	0.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethylformamide (DMF)	90 % v/v	100.0	0.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5 % v/v	100.0	97.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10 % v/v	100.0	96.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15 % v/v	100.0	98.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20 % v/v	100.0	103.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25 % v/v	100.0	139.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30 % v/v	100.0	175.0	%	Digital	Continuous	0.04M Britton-Robinson buffer	7	37°C	? mM 4-Methylumbelliferyl oleate	4-Methylumbelliferone , Oleic acid	—	—	Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethylformamide (DMF)

5 % v/v

100.0

103.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethylformamide (DMF)

10 % v/v

100.0

109.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethylformamide (DMF)

15 % v/v

100.0

150.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethylformamide (DMF)

20 % v/v

100.0

186.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethylformamide (DMF)

25 % v/v

100.0

175.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethylformamide (DMF)

30 % v/v

100.0

96.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethylformamide (DMF)

35 % v/v

100.0

56.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethylformamide (DMF)

40 % v/v

100.0

44.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethylformamide (DMF)

45 % v/v

100.0

39.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethylformamide (DMF)

50 % v/v

100.0

34.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethylformamide (DMF)

60 % v/v

100.0

28.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethylformamide (DMF)

70 % v/v

100.0

13.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethylformamide (DMF)

80 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethylformamide (DMF)

90 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

5 % v/v

100.0

97.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100.0

96.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15 % v/v

100.0

98.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100.0

103.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25 % v/v

100.0

139.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30 % v/v

100.0

175.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

35 % v/v

100.0

200.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40 % v/v

100.0

178.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

45 % v/v

100.0

120.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50 % v/v

100.0

81.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60 % v/v

100.0

52.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

70 % v/v

100.0

39.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

80 % v/v

100.0

21.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

90 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Dimethoxyethane

2.5 % v/v

100.0

90.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Dimethoxyethane

5 % v/v

100.0

87.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Dimethoxyethane

7.5 % v/v

100.0

103.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Dimethoxyethane

10 % v/v

100.0

134.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Dimethoxyethane

12.5 % v/v

100.0

141.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Dimethoxyethane

15 % v/v

100.0

142.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Dimethoxyethane

17.5 % v/v

100.0

145.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Dimethoxyethane

20 % v/v

100.0

133.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Dimethoxyethane

22.5 % v/v

100.0

107.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Dimethoxyethane

25 % v/v

100.0

75.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Dimethoxyethane

30 % v/v

100.0

15.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Dimethoxyethane

35 % v/v

100.0

3.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Dimethoxyethane

40 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Dimethoxyethane

45 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Dimethoxyethane

50 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethoxymethane

5 % v/v

100.0

87.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethoxymethane

10 % v/v

100.0

65.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethoxymethane

15 % v/v

100.0

60.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethoxymethane

20 % v/v

100.0

51.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethoxymethane

25 % v/v

100.0

1.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethoxymethane

30 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethoxymethane

35 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethoxymethane

40 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethoxymethane

45 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Diethoxyethane

2 % v/v

100.0

118.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Diethoxyethane

4 % v/v

100.0

111.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Diethoxyethane

5 % v/v

100.0

103.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Diethoxyethane

7.5 % v/v

100.0

79.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Diethoxyethane

10 % v/v

100.0

68.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Diethoxyethane

12.5 % v/v

100.0

33.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Diethoxyethane

15 % v/v

100.0

7.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Diethoxyethane

17.5 % v/v

100.0

2.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Diethoxyethane

20 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Diethoxyethane

25 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Diethoxyethane

30 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Diethoxyethane

35 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Diethoxyethane

40 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Diethoxyethane

45 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,2-Diethoxyethane

50 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

2,2-Dimethoxypropane

2 % v/v

100.0

86.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

2,2-Dimethoxypropane

4 % v/v

100.0

65.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

2,2-Dimethoxypropane

6 % v/v

100.0

57.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

2,2-Dimethoxypropane

8 % v/v

100.0

54.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

2,2-Dimethoxypropane

10 % v/v

100.0

44.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

2,2-Dimethoxypropane

12 % v/v

100.0

28.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

2,2-Dimethoxypropane

14 % v/v

100.0

13.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

2,2-Dimethoxypropane

16 % v/v

100.0

3.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

2,2-Dimethoxypropane

20 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

2,2-Dimethoxypropane

25 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

2,2-Dimethoxypropane

30 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

2,2-Dimethoxypropane

35 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

2,2-Dimethoxypropane

40 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

2,2-Dimethoxypropane

50 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Anisole

2.5 % v/v

100.0

8.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Anisole

5 % v/v

100.0

1.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Anisole

10 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Anisole

15 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Anisole

20 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Anisole

25 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Anisole

30 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Anisole

35 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Anisole

40 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Anisole

45 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Anisole

50 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

5 % v/v

100.0

71.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

10 % v/v

100.0

45.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

15 % v/v

100.0

34.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

20 % v/v

100.0

26.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

25 % v/v

100.0

23.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

30 % v/v

100.0

19.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

35 % v/v

100.0

21.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

40 % v/v

100.0

24.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

45 % v/v

100.0

25.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

50 % v/v

100.0

25.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

60 % v/v

100.0

21.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

70 % v/v

100.0

7.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

80 % v/v

100.0

1.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Tetrahydrofuran (THF)

90 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,4-Dioxane

5 % v/v

100.0

85.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,4-Dioxane

10 % v/v

100.0

97.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,4-Dioxane

15 % v/v

100.0

185.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,4-Dioxane

20 % v/v

100.0

164.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,4-Dioxane

25 % v/v

100.0

103.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,4-Dioxane

30 % v/v

100.0

39.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,4-Dioxane

35 % v/v

100.0

16.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,4-Dioxane

40 % v/v

100.0

4.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,4-Dioxane

45 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,4-Dioxane

50 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,4-Dioxane

60 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,4-Dioxane

70 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,4-Dioxane

80 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,4-Dioxane

90 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,3-Dioxane

5 % v/v

100.0

50.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,3-Dioxane

10 % v/v

100.0

25.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,3-Dioxane

15 % v/v

100.0

14.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,3-Dioxane

20 % v/v

100.0

6.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,3-Dioxane

25 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,3-Dioxane

30 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,3-Dioxane

35 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,3-Dioxane

40 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,3-Dioxane

45 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,3-Dioxane

50 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,3-Dioxane

60 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,3-Dioxane

70 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,3-Dioxane

80 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1,3-Dioxane

90 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Methanol

10 % v/v

100.0

67.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Methanol

20 % v/v

100.0

46.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Methanol

30 % v/v

100.0

36.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Methanol

40 % v/v

100.0

7.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Methanol

50 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Methanol

60 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Methanol

70 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Ethanol

10 % v/v

100.0

62.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Ethanol

20 % v/v

100.0

55.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Ethanol

30 % v/v

100.0

4.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Ethanol

40 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Ethanol

50 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Ethanol

60 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Ethanol

70 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1-Propanol

10 % v/v

100.0

20.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1-Propanol

20 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1-Propanol

30 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1-Propanol

40 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1-Propanol

50 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1-Propanol

60 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1-Propanol

70 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1-Butanol

10 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1-Butanol

20 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1-Butanol

30 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1-Butanol

40 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1-Butanol

50 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1-Butanol

60 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

1-Butanol

70 % v/v

100.0

0.0

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in %)

Activity - Classical

Activity (in mmol/(min*mg) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethylformamide (DMF)

20 % v/v

4.5

8.2

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

Dimethyl Sulfoxide (DMSO)

30 % v/v

4.5

9.1

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

1,4-Dioxane

15 % v/v

4.5

8.3

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

1,2-Dimethoxyethane

15 % v/v

4.5

6.3

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

Dimethylformamide (DMF)

20 % v/v

4.6

9.3

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl palmitate

4-Methylumbelliferone, Palmitic Acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

Dimethyl Sulfoxide (DMSO)

30 % v/v

4.6

9.7

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl palmitate

4-Methylumbelliferone, Palmitic Acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

1,4-Dioxane

15 % v/v

4.6

8.2

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl palmitate

4-Methylumbelliferone, Palmitic Acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

1,2-Dimethoxyethane

15 % v/v

4.6

6.7

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl palmitate

4-Methylumbelliferone, Palmitic Acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

Dimethylformamide (DMF)

20 % v/v

3.9

1.8

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl nonanoate

4-Methylumbelliferone, Nonanoic acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

Dimethyl Sulfoxide (DMSO)

30 % v/v

3.9

1.6

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl nonanoate

4-Methylumbelliferone, Nonanoic acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

1,4-Dioxane

15 % v/v

3.9

1.8

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl nonanoate

4-Methylumbelliferone, Nonanoic acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

1,2-Dimethoxyethane

15 % v/v

3.9

3.0

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl nonanoate

4-Methylumbelliferone, Nonanoic acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

Dimethylformamide (DMF)

20 % v/v

3.5

0.1

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl heptanoate

4-Methylumbelliferone, Heptanoic Acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

Dimethyl Sulfoxide (DMSO)

30 % v/v

3.5

0.15

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl heptanoate

4-Methylumbelliferone, Heptanoic Acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

1,4-Dioxane

15 % v/v

3.5

0.2

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl heptanoate

4-Methylumbelliferone, Heptanoic Acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

1,2-Dimethoxyethane

15 % v/v

3.5

0.75

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 4-Methylumbelliferyl heptanoate

4-Methylumbelliferone, Heptanoic Acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

Dimethylformamide (DMF)

20 % v/v

4.4

5.9

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 1-Monocaprin

Capric acid, glycerol

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

Initial reaction rate (mmol/(min*mg) measured by fluorometry

Dimethyl Sulfoxide (DMSO)

35 % v/v

4.4

6.0

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 1-Monocaprin

Capric acid, glycerol

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

Initial reaction rate (mmol/(min*mg) measured by fluorometry

Dimethylformamide (DMF)

20 % v/v

1.4

1.8

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 2-Monocaprin

Capric acid, glycerol

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

Initial reaction rate (mmol/(min*mg) measured by fluorometry

Dimethyl Sulfoxide (DMSO)

35 % v/v

1.4

2.0

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 2-Monocaprin

Capric acid, glycerol

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

Initial reaction rate (mmol/(min*mg) measured by fluorometry

Dimethylformamide (DMF)

20 % v/v

2.7

4.4

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 1-Monoolein

glycerol, Oleic acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

Initial reaction rate (mmol/(min*mg) measured by fluorometry

Dimethyl Sulfoxide (DMSO)

35 % v/v

2.7

5.4

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 1-Monoolein

glycerol, Oleic acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

Initial reaction rate (mmol/(min*mg) measured by fluorometry

Dimethylformamide (DMF)

20 % v/v

0.9

1.3

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 2-Monoolein

glycerol, Oleic acid

—

Classical aqueous control (in mmol/(min*mg))

Activity - Classical

Initial reaction rate (mmol/(min*mg) measured by fluorometry

Dimethyl Sulfoxide (DMSO)

35 % v/v

0.9

1.6

mmol/(min*mg)

Digital

Continuous

0.04M Britton-Robinson buffer

37°C

? mM 2-Monoolein

glycerol, Oleic acid

—

Classical aqueous control (in mmol/(min*mg))

Stability - Incubation

Activity after incubation (6 days at 37°C) measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent

Dimethylformamide (DMF)

20 % v/v

95.0

93.0

Digital

Continuous

Incubation: 0.04M Britton-Robinson buffer, Assay: 0.04M Britton-Robinson buffer

Incubation: 7, Assay: 7

Incubation: 37°C, Assay: 37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Water-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in the presence of organic solvent

Stability - Incubation

1,4-Dioxane

30 % v/v

95.0

91.0

Digital

Continuous

Incubation: 0.04M Britton-Robinson buffer, Assay: 0.04M Britton-Robinson buffer

Incubation: 7, Assay: 7

Incubation: 37°C, Assay: 37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Water-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in the presence of organic solvent

Stability - Incubation

1,2-Dimethoxyethane

15 % v/v

95.0

91.0

Digital

Continuous

Incubation: 0.04M Britton-Robinson buffer, Assay: 0.04M Britton-Robinson buffer

Incubation: 7, Assay: 7

Incubation: 37°C, Assay: 37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Water-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in the presence of organic solvent

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

15 % v/v

95.0

88.0

Digital

Continuous

Incubation: 0.04M Britton-Robinson buffer, Assay: 0.04M Britton-Robinson buffer

Incubation: 7, Assay: 7

Incubation: 37°C, Assay: 37°C

? mM 4-Methylumbelliferyl oleate

4-Methylumbelliferone, Oleic acid

—

Water-incubated control (in %) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether assay and control were in the presence of organic solvent

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Lipase from Rhizomucor miehei

Enzyme Description

Sequence

Experimental Data (190 measurements)

Visualization : Activity — Classical

Visualization : Stability — Incubation

Structure

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