Lipase from Rhizomucor miehei

Enzyme Description

Species
Rhizomucor miehei β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 363 amino acids
MVLKQRANYLGFLIVFFTAFLVEAVPIKRQSNSTVDSLPPLIPSRTSAPSSSPSTTDPEAPAMSRNGPLPSDVETKYGMALNATSYPDSVVQAMSIDGGIRAATSQEINELTYYTTLSANSYCRTVIPGATWDCIHCDATEDLKIIKTWSTLIYDTNAMVARGDSEKTIYIVFRGSSSIRNWIADLTFVPVSYPPVSGTKVHKGFLDSYGEVQNELVATVLDQFKQYPSYKVAVTGHSLGGATALLCALDLYQREEGLSSSNLFLYTQGQPRVGDPAFANYVVSTGIPYRRTVNERDIVPHLPPAAFGFLHAGEEYWITDNSPETVQVCTSDLETSDCSNSIVPFTSVLDHLSYFGINTGLCT
Wakako Tsuzuki et al. (2003) β€” Polar Organic Solvent Added to an Aqueous Solution Changes Hydrolytic Property of Lipase
Bioscience, Biotechnology, and Biochemistry  Β· doi:10.1271/bbb.67.1660 β†—  Β· Activity - Classical Stability - Incubation
190 measurements ▼
Database ID
UniProt: P19515 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased

Experimental Data (190 measurements)

190 measurements β€” page 3 of 10
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Dimethoxyethane 40 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Dimethoxyethane 45 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Dimethoxyethane 50 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent Dimethoxymethane 5 % v/v 100.0 87.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent Dimethoxymethane 10 % v/v 100.0 65.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent Dimethoxymethane 15 % v/v 100.0 60.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent Dimethoxymethane 20 % v/v 100.0 51.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent Dimethoxymethane 25 % v/v 100.0 1.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent Dimethoxymethane 30 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent Dimethoxymethane 35 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent Dimethoxymethane 40 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent Dimethoxymethane 45 % v/v 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Diethoxyethane 2 % v/v 100.0 118.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Diethoxyethane 4 % v/v 100.0 111.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Diethoxyethane 5 % v/v 100.0 103.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Diethoxyethane 7.5 % v/v 100.0 79.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Diethoxyethane 10 % v/v 100.0 68.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Diethoxyethane 12.5 % v/v 100.0 33.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Diethoxyethane 15 % v/v 100.0 7.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Diethoxyethane 17.5 % v/v 100.0 2.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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