Lipase from Rhizomucor miehei

Enzyme Description

Species
Rhizomucor miehei β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 363 amino acids
MVLKQRANYLGFLIVFFTAFLVEAVPIKRQSNSTVDSLPPLIPSRTSAPSSSPSTTDPEAPAMSRNGPLPSDVETKYGMALNATSYPDSVVQAMSIDGGIRAATSQEINELTYYTTLSANSYCRTVIPGATWDCIHCDATEDLKIIKTWSTLIYDTNAMVARGDSEKTIYIVFRGSSSIRNWIADLTFVPVSYPPVSGTKVHKGFLDSYGEVQNELVATVLDQFKQYPSYKVAVTGHSLGGATALLCALDLYQREEGLSSSNLFLYTQGQPRVGDPAFANYVVSTGIPYRRTVNERDIVPHLPPAAFGFLHAGEEYWITDNSPETVQVCTSDLETSDCSNSIVPFTSVLDHLSYFGINTGLCT
Wakako Tsuzuki et al. (2003) β€” Polar Organic Solvent Added to an Aqueous Solution Changes Hydrolytic Property of Lipase
Bioscience, Biotechnology, and Biochemistry  Β· doi:10.1271/bbb.67.1660 β†—  Β· Activity - Classical Stability - Incubation
190 measurements ▼
Database ID
UniProt: P19515 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased

Experimental Data (190 measurements)

190 measurements β€” page 4 of 10
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Extraction method Data type Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Diethoxyethane 20 % (v/v) 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Diethoxyethane 25 % (v/v) 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Diethoxyethane 30 % (v/v) 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Diethoxyethane 35 % (v/v) 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Diethoxyethane 40 % (v/v) 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Diethoxyethane 45 % (v/v) 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 1,2-Diethoxyethane 50 % (v/v) 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 2,2-Dimethoxypropane 2 % (v/v) 100.0 86.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 2,2-Dimethoxypropane 4 % (v/v) 100.0 65.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 2,2-Dimethoxypropane 6 % (v/v) 100.0 57.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 2,2-Dimethoxypropane 8 % (v/v) 100.0 54.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 2,2-Dimethoxypropane 10 % (v/v) 100.0 44.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 2,2-Dimethoxypropane 12 % (v/v) 100.0 28.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 2,2-Dimethoxypropane 14 % (v/v) 100.0 13.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 2,2-Dimethoxypropane 16 % (v/v) 100.0 3.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 2,2-Dimethoxypropane 20 % (v/v) 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 2,2-Dimethoxypropane 25 % (v/v) 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 2,2-Dimethoxypropane 30 % (v/v) 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 2,2-Dimethoxypropane 35 % (v/v) 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-Methylumbelliferone fluorescence measurement, excitation at 325 nm, absorbance measurement at 450 nm) in the presence of organic solvent 2,2-Dimethoxypropane 40 % (v/v) 100.0 0.0 % Digital Continuous 0.04M Britton-Robinson buffer 7 37Β°C ? mM 4-Methylumbelliferyl oleate 4-Methylumbelliferone , Oleic acid β€” β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

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Visualization : Stability β€” Incubation

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Structure

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