Lipase (gene lip9) from Pseudomonas aeruginosa LST-03

Enzyme Description

Species

Extremophile

EC Number

3.1.1.3 ↗

Sequence

Length: 311 amino acids

MKKKSLLPLGLAIGLASLAASPLIQASTYTQTKYPIVLAHGMLGFDNILGVDYWFGIPSALRRDGAQVYVTEVSQLDTSEVRGEQLLQQVEEIVALSGQPKVNLIGHSHGGPTIRYVAAVRPDLIASATSVGAPHKGSDTADFLRQIPPGSAGEAILSGLVNSLGALISFLSSGSTGTQNSLGSLESLNSEGAARFNAKYPQGIPTSACGEGAYKVNGVSYYSWSGSSPLTNFLDPSDAFLGASSLTFKNGTANDGLVGTCSSHLGMVIRDNYRMNHLDEVNQVFGLTSLFETSPVSVYRQHANRLKNASL

Hiroyasu Ogino et al. (2000) — Purification and characterization of organic solvent-stable lipase from organic solvent-tolerant Pseudomonas aeruginosa LST-03

Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(00)89095-7 ↗ · Activity - Classical Stability - Half-life

43 measurements ▼

Database ID

UniProt: A8QYB2 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, bacterium Pseudomonas aeruginosa LST-03

Experimental Data (43 measurements)

43 measurements — page 1 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	n-Octane	50 % (v/v)	1.0	1.01	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	Chloroform	50 % (v/v)	1.0	0.1	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	1-Hexanol	50 % (v/v)	1.0	0.11	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	1-Pentanol	50 % (v/v)	1.0	0.12	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	1-Heptanol	50 % (v/v)	1.0	0.13	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	1-Decanol	50 % (v/v)	1.0	0.14	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	1-Octanol	50 % (v/v)	1.0	0.15	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	1-Butanol	50 % (v/v)	1.0	0.16	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	Benzene	50 % (v/v)	1.0	0.19	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	Ethanol	50 % (v/v)	1.0	0.21	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	Toluene	50 % (v/v)	1.0	0.23	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	Methanol	50 % (v/v)	1.0	0.25	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	Isopropanol	50 % (v/v)	1.0	0.28	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	p-Xylene	50 % (v/v)	1.0	0.46	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	Acetone	50 % (v/v)	1.0	0.35	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	n-Decane	50 % (v/v)	1.0	1.1	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50 % (v/v)	1.0	0.99	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	Dimethylformamide (DMF)	50 % (v/v)	1.0	0.92	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	n-Heptane	50 % (v/v)	1.0	0.84	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)
Activity - Classical	Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent	n-Dodecane	50 % (v/v)	1.0	0.73	relative units	Direct	Continuous	50 mM McIlvain buffer	7	30°C	2g/20 ml (including organic solvent) Tributyrin	Butyric Acid , Glycerol	—	700 rpm	Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

n-Octane

50 % (v/v)

1.0

1.01

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Chloroform

50 % (v/v)

1.0

0.1

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

1-Hexanol

50 % (v/v)

1.0

0.11

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

1-Pentanol

50 % (v/v)

1.0

0.12

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

1-Heptanol

50 % (v/v)

1.0

0.13

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

1-Decanol

50 % (v/v)

1.0

0.14

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

1-Octanol

50 % (v/v)

1.0

0.15

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

1-Butanol

50 % (v/v)

1.0

0.16

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Benzene

50 % (v/v)

1.0

0.19

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Ethanol

50 % (v/v)

1.0

0.21

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Toluene

50 % (v/v)

1.0

0.23

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Methanol

50 % (v/v)

1.0

0.25

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Isopropanol

50 % (v/v)

1.0

0.28

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

p-Xylene

50 % (v/v)

1.0

0.46

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Acetone

50 % (v/v)

1.0

0.35

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

n-Decane

50 % (v/v)

1.0

1.1

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50 % (v/v)

1.0

0.99

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Dimethylformamide (DMF)

50 % (v/v)

1.0

0.92

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

n-Heptane

50 % (v/v)

1.0

0.84

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

n-Dodecane

50 % (v/v)

1.0

0.73

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Cyclohexane

50 % (v/v)

1.0

0.71

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Isooctane

50 % (v/v)

1.0

0.65

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

n-Hexane

50 % (v/v)

1.0

0.57

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

n-Hexadecane

50 % (v/v)

1.0

0.57

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

n-Tetradecane

50 % (v/v)

1.0

0.47

relative units

Direct

Continuous

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, Glycerol

—

700 rpm

Classical aqueous control (in relative units)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

tert-Butanol

25 % (v/v)

12.5

1.2

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

1,5-Pentanediol

25 % (v/v)

12.5

6.8

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Ethanol

25 % (v/v)

12.5

7.6

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Triethylene Glycol

25 % (v/v)

12.5

7.7

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

p-Xylene

25 % (v/v)

12.5

8.3

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Toluene

25 % (v/v)

12.5

9.0

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

1,4-Butanediol

25 % (v/v)

12.5

9.3

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Dimethylformamide (DMF)

25 % (v/v)

12.5

9.5

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Glycerol

25 % (v/v)

12.5

10.8

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Methanol

25 % (v/v)

12.5

11.3

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Hexane

25 % (v/v)

12.5

11.6

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Cyclohexane

25 % (v/v)

12.5

15.7

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Isooctane

25 % (v/v)

12.5

16.4

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Heptane

25 % (v/v)

12.5

17.7

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Octane

25 % (v/v)

12.5

29.0

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Dimethyl Sulfoxide (DMSO)

25 % (v/v)

12.5

36.2

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Ethylene Glycol

25 % (v/v)

12.5

50.0

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Decane

25 % (v/v)

12.5

>100.0

days

Direct

Continuous

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Visualization : Stability — Half-life

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Structure

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Lipase (gene lip9) from Pseudomonas aeruginosa LST-03

Enzyme Description

Sequence

Experimental Data (43 measurements)

Visualization : Activity — Classical

Visualization : Stability — Half-life

Structure

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