Feruloyl esterase B (gene fae-1) from Neurospora crassa

Enzyme Description

Species

Extremophile

EC Number

3.1.1.73 ↗

Sequence

Length: 292 amino acids

MLPRTLLGLALTAATGLCASLQQVTNWGSNPTNIRMYTYVPDKLATKPAIIVALHGCGGTAPSWYSGTRLPSYADQYGFILIYPGTPNMSNCWGVNDPASLTHGAGGDSLGIVAMVNYTIAKYNADASRVYVMGTSSGGMMTNVMAATYPEVFEAGAAYSGVAHACFAGAASATPFSPNQTCARGLQHTPEEWGNFVRNSYPGYTGRRPRMQIYHGLADNLVYPRCAMEALKQWSNVLGVEFSRNVSGVPSQAYTQIVYGDGSKLVGYMGAGVGHVAPTNEQVMLKFFGLIN

Craig B. Faulds et al. (2011) — Influence of organic co-solvents on the activity and substrate specificity of feruloyl esterases

Bioresource Technology · doi:10.1016/j.biortech.2011.01.088 ↗ · Activity - Classical Stability - C50 Stability - Incubation Activity + Stability - Incubation

63 measurements ▼

Database ID

UniProt: Q9HGR3 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Recombinant, host yeast Pichia pastoris

Experimental Data (63 measurements)

63 measurements — page 2 of 4

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Extraction method	Data type	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20 % v/v	100.0	100.0	%	Digital	Continuous	100 mM MOPS	6	37°C	1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25 % v/v	100.0	99.0	%	Digital	Continuous	100 mM MOPS	6	37°C	1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30 % v/v	100.0	85.0	%	Digital	Continuous	100 mM MOPS	6	37°C	1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	35 % v/v	100.0	62.0	%	Digital	Continuous	100 mM MOPS	6	37°C	1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40 % v/v	100.0	18.0	%	Digital	Continuous	100 mM MOPS	6	37°C	1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	Acetone	5 % v/v	100.0	25.0	%	Digital	Continuous	100 mM MOPS	6	37°C	60 µM Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1 % v/v	100.0	89.0	%	Digital	Continuous	100 mM MOPS	6	37°C	60 µM Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10 % v/v	100.0	48.0	%	Digital	Continuous	100 mM MOPS	6	37°C	60 µM Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15 % v/v	100.0	33.0	%	Digital	Continuous	100 mM MOPS	6	37°C	60 µM Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20 % v/v	100.0	23.0	%	Digital	Continuous	100 mM MOPS	6	37°C	60 µM Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25 % v/v	100.0	17.0	%	Digital	Continuous	100 mM MOPS	6	37°C	60 µM Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30 % v/v	100.0	4.0	%	Digital	Continuous	100 mM MOPS	6	37°C	60 µM Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40 % v/v	100.0	0.0	%	Digital	Continuous	100 mM MOPS	6	37°C	60 µM Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50 % v/v	100.0	0.0	%	Digital	Continuous	100 mM MOPS	6	37°C	60 µM Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	Acetone	1 % v/v	100.0	81.0	%	Digital	Continuous	100 mM MOPS	6	37°C	60 µM Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15 % v/v	100.0	84.0	%	Digital	Continuous	100 mM MOPS	6	37°C	60 µM Methyl p-coumarate	Methanol , p-Coumaric acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	Acetone	10 % v/v	100.0	16.0	%	Digital	Continuous	100 mM MOPS	6	37°C	60 µM Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	Acetone	15 % v/v	100.0	11.0	%	Digital	Continuous	100 mM MOPS	6	37°C	60 µM Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent	1,4-Dioxane	5 % v/v	100.0	26.0	%	Digital	Continuous	100 mM MOPS	6	37°C	60 µM Methyl ferulate	Ferulic acid , Methanol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10 % v/v	100.0	97.0	%	Digital	Continuous	100 mM MOPS	6	37°C	60 µM Methyl p-coumarate	Methanol , p-Coumaric acid	—	—	Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

25 % v/v

100.0

40.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100.0

88.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25 % v/v

100.0

111.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30 % v/v

100.0

66.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

35 % v/v

100.0

41.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40 % v/v

100.0

34.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

1 % v/v

100.0

104.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

5 % v/v

100.0

85.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

10 % v/v

100.0

74.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

15 % v/v

100.0

69.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

20 % v/v

100.0

56.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

5 % v/v

100.0

69.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

30 % v/v

100.0

16.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

40 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

1 % v/v

100.0

132.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

5 % v/v

100.0

142.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100.0

130.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15 % v/v

100.0

134.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25 % v/v

100.0

99.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30 % v/v

100.0

85.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

35 % v/v

100.0

62.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40 % v/v

100.0

18.0

Digital

Continuous

100 mM MOPS

37°C

1 mM p-Nitrophenol acetate

Acetic Acid, p-Nitrophenol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

5 % v/v

100.0

25.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

1 % v/v

100.0

89.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100.0

48.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15 % v/v

100.0

33.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20 % v/v

100.0

23.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

25 % v/v

100.0

17.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30 % v/v

100.0

4.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

1 % v/v

100.0

81.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

15 % v/v

100.0

84.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

10 % v/v

100.0

16.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

15 % v/v

100.0

11.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1,4-Dioxane

5 % v/v

100.0

26.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

100.0

97.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

5 % v/v

100.0

75.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

1 % v/v

100.0

68.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1,4-Dioxane

30 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1,4-Dioxane

25 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1,4-Dioxane

20 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1,4-Dioxane

15 % v/v

100.0

5.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1,4-Dioxane

10 % v/v

100.0

12.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

20 % v/v

100.0

5.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1,4-Dioxane

1 % v/v

100.0

66.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

25 % v/v

100.0

0.0

Digital

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Classical aqueous control (in %)

Activity + Stability - Incubation

Activity after incubation (96 hours at 37°C) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

4.66

3.48

U/mg

Direct

Continuous

Incubation: 100 mM MOPS, Assay: 100 mM MOPS

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Non-incubated control (in U/mg), assay in the presence of organic solvent | Clear about assay in the presence of organic solvent and control in aqueous phase

Activity + Stability - Incubation

Activity after incubation (96 hours at 37°C) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

10 % v/v

4.66

3.29

U/mg

Direct

Continuous

Incubation: 100 mM MOPS, Assay: 100 mM MOPS

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Non-incubated control (in U/mg), assay in the presence of organic solvent | Clear about assay in the presence of organic solvent and control in aqueous phase

Activity + Stability - Incubation

Activity after incubation (96 hours at 37°C) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

42.12

28.88

U/mg

Direct

Continuous

Incubation: 100 mM MOPS, Assay: 100 mM MOPS

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Non-incubated control (in U/mg), assay in the presence of organic solvent | Clear about assay in the presence of organic solvent and control in aqueous phase

Activity + Stability - Incubation

Activity after incubation (96 hours at 37°C) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

10 % v/v

42.12

23.31

U/mg

Direct

Continuous

Incubation: 100 mM MOPS, Assay: 100 mM MOPS

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Non-incubated control (in U/mg), assay in the presence of organic solvent | Clear about assay in the presence of organic solvent and control in aqueous phase

Stability - C50

Concentration (in % v/v) for which 50% of enzyme activity is lost, as measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

—

22.0

% (v/v)

Direct

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Not applicable control (in % (v/v))

Stability - C50

Concentration (in % v/v) for which 50% of enzyme activity is lost, as measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

1,4-Dioxane

—

3.0

% (v/v)

Direct

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Not applicable control (in % (v/v))

Stability - C50

Concentration (in % v/v) for which 50% of enzyme activity is lost, as measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

—

4.0

% (v/v)

Direct

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Not applicable control (in % (v/v))

Stability - C50

Concentration (in % v/v) for which 50% of enzyme activity is lost, as measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

—

34.0

% (v/v)

Direct

Continuous

100 mM MOPS

37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Not applicable control (in % (v/v))

Stability - C50

Concentration (in % v/v) for which 50% of enzyme activity is lost, as measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

—

10.0

% (v/v)

Direct

Continuous

100 mM MOPS

37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Not applicable control (in % (v/v))

Stability - Incubation

Activity after incubation (96 hours at 37°C) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

4.41

3.48

U/mg

Direct

Continuous

Incubation: 100 mM MOPS, Assay: 100 mM MOPS

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Non-incubated control (in U/mg) in the presence of organic solvent (1 minute incubation, 5% DMSO), assay in the presence of organic solvent | Clear about assay in the presence of organic solvent and control in the presence of organic solvent

Stability - Incubation

Activity after incubation (96 hours at 37°C) measured by absorbance spectrophotometry (ferulic acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

10 % v/v

4.35

3.29

U/mg

Direct

Continuous

Incubation: 100 mM MOPS, Assay: 100 mM MOPS

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

60 µM Methyl ferulate

Ferulic acid, Methanol

—

Non-incubated control (in U/mg) in the presence of organic solvent (1 minute incubation, 5% acetone), assay in the presence of organic solvent | Clear about assay in the presence of organic solvent and control in the presence of organic solvent

Stability - Incubation

Activity after incubation (96 hours at 37°C) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10 % v/v

36.7

28.88

U/mg

Direct

Continuous

Incubation: 100 mM MOPS, Assay: 100 mM MOPS

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

Stability - Incubation

Activity after incubation (96 hours at 37°C) measured by absorbance spectrophotometry (p-coumaric acid absorbance measurement, 335 nm) in the presence of organic solvent

Acetone

10 % v/v

39.4

23.31

U/mg

Direct

Continuous

Incubation: 100 mM MOPS, Assay: 100 mM MOPS

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

60 µM Methyl p-coumarate

Methanol, p-Coumaric acid

—

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity + Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — C₅₀

One bar per measurement. Colour = solvent, shade = temperature. Hover for details.

Structure

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Feruloyl esterase B (gene fae-1) from Neurospora crassa

Enzyme Description

Sequence

Experimental Data (63 measurements)

Visualization : Activity — Classical

Visualization : Stability — Incubation

Visualization : Activity + Stability — Incubation

Visualization : Stability — C50

Structure

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Visualization : Stability — C₅₀